Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4060580..4061199 Replicon chromosome
Accession NZ_CP046645
Organism Klebsiella pneumoniae strain EGKP3

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GPU01_RS19680 Protein ID WP_002892050.1
Coordinates 4060981..4061199 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag GPU01_RS19675 Protein ID WP_002892066.1
Coordinates 4060580..4060954 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPU01_RS19665 (GPU01_19360) 4055732..4056925 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GPU01_RS19670 (GPU01_19365) 4056948..4060094 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
GPU01_RS19675 (GPU01_19370) 4060580..4060954 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
GPU01_RS19680 (GPU01_19375) 4060981..4061199 + 219 WP_002892050.1 HHA domain-containing protein Toxin
GPU01_RS19685 (GPU01_19380) 4061358..4061924 + 567 WP_002892030.1 maltose O-acetyltransferase -
GPU01_RS19690 4061896..4062036 - 141 WP_032409038.1 hypothetical protein -
GPU01_RS19695 (GPU01_19385) 4062057..4062527 + 471 WP_002892026.1 YlaC family protein -
GPU01_RS19700 (GPU01_19390) 4062502..4063953 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
GPU01_RS19705 (GPU01_19395) 4064054..4064752 + 699 WP_004893822.1 GNAT family protein -
GPU01_RS19710 (GPU01_19400) 4064749..4064889 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
GPU01_RS19715 (GPU01_19405) 4064889..4065152 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T144649 WP_002892050.1 NZ_CP046645:4060981-4061199 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T144649 NZ_CP062211:c993479-993261 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT144649 WP_002892066.1 NZ_CP046645:4060580-4060954 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT144649 NZ_CP062211:c993879-993505 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References