Detailed information of TA system TA144647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2723403..2724991
• Replicon: chromosome
• Accession: NZ_CP046645
• Organism: Klebsiella pneumoniae strain EGKP3

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A9J6RYF8
• Locus tag: GPU01_RS13285
• Protein ID: WP_004892016.1
• Coordinates: 2723403..2724725 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GPU01_RS13290
• Protein ID: WP_004176198.1
• Coordinates: 2724725..2724991 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPU01_RS13270 (GPU01_13080)	2718459..2720960	+	2502	WP_004892009.1	fimbria/pilus outer membrane usher protein	-
GPU01_RS13275 (GPU01_13085)	2720948..2721952	+	1005	WP_004892012.1	fimbrial protein	-
GPU01_RS13280 (GPU01_13090)	2722094..2723386	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
GPU01_RS13285 (GPU01_13095)	2723403..2724725	-	1323	WP_004892016.1	type II toxin-antitoxin system HipA family toxin	Toxin
GPU01_RS13290 (GPU01_13100)	2724725..2724991	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GPU01_RS13295 (GPU01_13105)	2725256..2725582	-	327	WP_004892019.1	hydroxyisourate hydrolase	-
GPU01_RS13300 (GPU01_13110)	2725579..2726079	-	501	WP_004892023.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
GPU01_RS13305 (GPU01_13115)	2726057..2727436	-	1380	WP_004176204.1	nucleobase:cation symporter-2 family protein	-
GPU01_RS13310 (GPU01_13120)	2727782..2728936	+	1155	WP_004892025.1	FAD-dependent urate hydroxylase HpxO	-
GPU01_RS13315 (GPU01_13125)	2728917..2729840	-	924	WP_004892028.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2520942..2760474	239532

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49141.44 Da
• Isoelectric Point: 7.5357

>T144647 WP_004892016.1 NZ_CP046645:c2724725-2723403 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T144647 NZ_CP062211:c884829-884551 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT144647 WP_004176198.1 NZ_CP046645:c2724991-2724725 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT144647 NZ_CP062211:c885092-884832 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA