Detailed information of TA system TA144630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5152066..5152706
• Replicon: chromosome
• Accession: NZ_CP046621
• Organism: Pseudomonas alkylphenolica strain Neo

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GPJ81_RS23365
• Protein ID: WP_157194384.1
• Coordinates: 5152296..5152706 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GPJ81_RS23360
• Protein ID: WP_157194383.1
• Coordinates: 5152066..5152296 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPJ81_RS23340	5147522..5148874	-	1353	WP_157194379.1	precorrin-3B synthase	-
GPJ81_RS23345	5148977..5150188	+	1212	WP_157194380.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
GPJ81_RS23350	5150181..5151278	+	1098	WP_157194381.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
GPJ81_RS23355	5151275..5152006	+	732	WP_157194382.1	cobalt-precorrin-6A reductase	-
GPJ81_RS23360	5152066..5152296	+	231	WP_157194383.1	antitoxin	Antitoxin
GPJ81_RS23365	5152296..5152706	+	411	WP_157194384.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GPJ81_RS23370	5152859..5153296	+	438	WP_157194385.1	NfeD family protein	-
GPJ81_RS23375	5153320..5154177	+	858	WP_038615104.1	SPFH/Band 7/PHB domain protein	-
GPJ81_RS23380	5154297..5154683	+	387	WP_157194386.1	DUF2946 domain-containing protein	-
GPJ81_RS23385	5154731..5155201	+	471	WP_157194387.1	copper chaperone PCu(A)C	-
GPJ81_RS23390	5155221..5155655	+	435	WP_157194388.1	DUF2946 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15184.49 Da
• Isoelectric Point: 6.9685

>T144630 WP_157194384.1 NZ_CP046621:5152296-5152706 [Pseudomonas alkylphenolica]
MLKFMLDTNICIFTLKNKPEEIREAFNRHHGQLCISTITLMELIYGAEKSAVPERNLSVIEGFAARLEVLNYDSHAAAHS
GQLRAELARAGTPVGPYDQMIAGHARSLGLVLVSNNLREFQRVPGLRVEDWLTPVN

Nucleotide

• Length: 411 bp

>T144630 NZ_CP062204:2792878-2792985 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.47 Da
• Isoelectric Point: 4.3867

>AT144630 WP_157194383.1 NZ_CP046621:5152066-5152296 [Pseudomonas alkylphenolica]
MDQGAVFKSNRSQAIRLPKSVALPEDITRVDVVAIGRTRIISPAGEAWDNWFEGECVSPDFMASREQPADQEREGF

Nucleotide

• Length: 231 bp

>AT144630 NZ_CP062204:c2792831-2792765 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT