Detailed information of TA system TA144628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2494925..2496138
• Replicon: chromosome
• Accession: NZ_CP046621
• Organism: Pseudomonas alkylphenolica strain Neo

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GPJ81_RS11310
• Protein ID: WP_157192248.1
• Coordinates: 2494925..2495494 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GPJ81_RS11315
• Protein ID: WP_157192249.1
• Coordinates: 2495581..2496138 (-)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPJ81_RS11285	2490381..2491055	+	675	WP_157194720.1	tRNA (adenine(22)-N(1))-methyltransferase TrmK	-
GPJ81_RS11290	2491081..2491785	-	705	WP_157192244.1	TIGR02594 family protein	-
GPJ81_RS11295	2491888..2492820	-	933	WP_157192245.1	peptidoglycan-binding protein	-
GPJ81_RS11300	2493133..2494233	+	1101	WP_157192246.1	DUF3616 domain-containing protein	-
GPJ81_RS11305	2494240..2494641	-	402	WP_157192247.1	hypothetical protein	-
GPJ81_RS11310	2494925..2495494	-	570	WP_157192248.1	HD domain-containing protein	Toxin
GPJ81_RS11315	2495581..2496138	-	558	WP_157192249.1	helix-turn-helix domain-containing protein	Antitoxin
GPJ81_RS11320	2496341..2496682	-	342	WP_157192250.1	DUF3077 domain-containing protein	-
GPJ81_RS11325	2497019..2499553	-	2535	WP_198325412.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (9-147)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-46)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21433.59 Da
• Isoelectric Point: 5.1680

>T144628 WP_157192248.1 NZ_CP046621:c2495494-2494925 [Pseudomonas alkylphenolica]
MDIEKIKGRLEFLRQAEKLKDVLRSAHTSCGRTESTAEHSWRLCLMAIVFEDQLPRLDVLKMLKMCVIHDLGEAINGDIP
AVSKAAFPNKSEQERNDLLLLTSALDEPLKAEILALWDDYENARSAEAKAVKALDKLETILQHNQGKNPPGFDYQFNLAY
GKKYTDADPLFESLRSILDEDTKEKMIKA

Nucleotide

• Length: 570 bp

>T144628 NZ_CP062204:2124322-2124425 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20580.76 Da
• Isoelectric Point: 8.5505

>AT144628 WP_157192249.1 NZ_CP046621:c2496138-2495581 [Pseudomonas alkylphenolica]
MPDMLLKPALPGVALRRWRLLHRVKQNHAAELFNVTQSTISRWESGVQAMDPAAHKRLEQLLAARLDAAADQVLARLVRE
SPRPIHLICDLTHRLLACSSARAAEFTLPVSELLGRSLWRFATPEIIGNELALDESGWRETCAPPALEFTTGNNHSALVP
ISQSRCRWTRIPLSDGTAARLVETL

Nucleotide

• Length: 558 bp

>AT144628 NZ_CP062204:c2124463-2124192 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT