Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-RHH
Location 4671494..4672110 Replicon chromosome
Accession NZ_CP046618
Organism Enterobacter asburiae strain AEB30

Toxin (Protein)


Gene name vapC Uniprot ID W7NIR0
Locus tag EAAEB30_RS22250 Protein ID WP_032648446.1
Coordinates 4671494..4671865 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1H8XBY4
Locus tag EAAEB30_RS22255 Protein ID WP_045909092.1
Coordinates 4671868..4672110 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAAEB30_RS22235 4668994..4669896 + 903 WP_008501831.1 formate dehydrogenase subunit beta -
EAAEB30_RS22240 4669893..4670528 + 636 WP_008501832.1 formate dehydrogenase cytochrome b556 subunit -
EAAEB30_RS22245 4670525..4671454 + 930 WP_008501833.1 formate dehydrogenase accessory protein FdhE -
EAAEB30_RS22250 4671494..4671865 - 372 WP_032648446.1 type II toxin-antitoxin system VapC family toxin Toxin
EAAEB30_RS22255 4671868..4672110 - 243 WP_045909092.1 ribbon-helix-helix protein, CopG family Antitoxin
EAAEB30_RS22260 4672309..4673223 + 915 WP_157189887.1 alpha/beta hydrolase -
EAAEB30_RS22265 4673238..4674179 - 942 WP_023309755.1 fatty acid biosynthesis protein FabY -
EAAEB30_RS22270 4674224..4674661 - 438 WP_008501836.1 D-tyrosyl-tRNA(Tyr) deacylase -
EAAEB30_RS22275 4674658..4675539 - 882 WP_032648442.1 virulence factor BrkB family protein -
EAAEB30_RS22280 4675533..4676132 - 600 WP_024909213.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-109)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-49)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13700.81 Da        Isoelectric Point: 6.9790

>T144625 WP_032648446.1 NZ_CP046618:c4671865-4671494 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAANTIENTASHRSISLITWMEVMVGARKHGHEAKTASVMGAFEIIDVTREIAERSVVLR
EKHGMKLPDAIILATAQSRNCPLITRNTKDFQGIPGVVSPYQL

Download         Length: 372 bp

>T144625 NZ_CP062204:1423170-1423568 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9440.63 Da        Isoelectric Point: 4.2584

>AT144625 WP_045909092.1 NZ_CP046618:c4672110-4671868 [Enterobacter asburiae]
MSLLAELNMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Download         Length: 243 bp

>AT144625 NZ_CP062204:1422943-1423170 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W7NIR0

Antitoxin

Source ID Structure
AlphaFold DB A0A1H8XBY4

References