Detailed information of TA system TA144624

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3923099..3923756
• Replicon: chromosome
• Accession: NZ_CP046618
• Organism: Enterobacter asburiae strain AEB30

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: W7P4L3
• Locus tag: EAAEB30_RS18520
• Protein ID: WP_023326024.1
• Coordinates: 3923099..3923509 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W7NX65
• Locus tag: EAAEB30_RS18525
• Protein ID: WP_006178375.1
• Coordinates: 3923490..3923756 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAAEB30_RS18500	3919092..3920825	-	1734	WP_024906585.1	single-stranded-DNA-specific exonuclease RecJ	-
EAAEB30_RS18505	3920831..3921544	-	714	WP_157189696.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
EAAEB30_RS18510	3921573..3922469	-	897	WP_157189697.1	site-specific tyrosine recombinase XerD	-
EAAEB30_RS18515	3922571..3923092	+	522	WP_023309099.1	flavodoxin FldB	-
EAAEB30_RS18520	3923099..3923509	-	411	WP_023326024.1	protein YgfX	Toxin
EAAEB30_RS18525	3923490..3923756	-	267	WP_006178375.1	FAD assembly factor SdhE	Antitoxin
EAAEB30_RS18530	3924051..3925031	+	981	WP_157189698.1	tRNA-modifying protein YgfZ	-
EAAEB30_RS18535	3925131..3925790	-	660	WP_024906580.1	hemolysin III family protein	-
EAAEB30_RS18540	3926056..3926787	+	732	WP_032650129.1	MurR/RpiR family transcriptional regulator	-
EAAEB30_RS18545	3926904..3928337	+	1434	WP_024906578.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16063.06 Da
• Isoelectric Point: 10.9468

>T144624 WP_023326024.1 NZ_CP046618:c3923509-3923099 [Enterobacter asburiae]
VVLWQSDLRVSWRSQWMSLLLHGLVAAIVLLMPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGK
EWDILGMPWMLNCGMMLRLRKVDGGRCQHLWLAADSMDAAEWRDLRRMLLQQTTQG

Nucleotide

• Length: 411 bp

>T144624 NZ_CP062204:1086214-1086549 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10569.05 Da
• Isoelectric Point: 5.1340

>AT144624 WP_006178375.1 NZ_CP046618:c3923756-3923490 [Enterobacter asburiae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDDDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT144624 NZ_CP062204:1085966-1086214 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: W7P4L3

Antitoxin

• AlphaFold DB: W7NX65