Detailed information of TA system TA144614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1084984..1085604
• Replicon: chromosome
• Accession: NZ_CP046618
• Organism: Enterobacter asburiae strain AEB30

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: EAAEB30_RS05020
• Protein ID: WP_157188699.1
• Coordinates: 1084984..1085202 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V3PBI9
• Locus tag: EAAEB30_RS05025
• Protein ID: WP_008499288.1
• Coordinates: 1085230..1085604 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAAEB30_RS04990	1080998..1081258	+	261	WP_006176933.1	type B 50S ribosomal protein L31	-
EAAEB30_RS04995	1081261..1081401	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
EAAEB30_RS05000	1081398..1082108	-	711	WP_024906842.1	GNAT family N-acetyltransferase	-
EAAEB30_RS05005	1082210..1083670	+	1461	WP_157188698.1	PLP-dependent aminotransferase family protein	-
EAAEB30_RS05010	1083642..1084109	-	468	WP_024906844.1	membrane protein	-
EAAEB30_RS05015	1084227..1084778	-	552	WP_024906845.1	maltose O-acetyltransferase	-
EAAEB30_RS05020	1084984..1085202	-	219	WP_157188699.1	hemolysin expression modulator Hha	Toxin
EAAEB30_RS05025	1085230..1085604	-	375	WP_008499288.1	Hha toxicity modulator TomB	Antitoxin
EAAEB30_RS05030	1086114..1089260	-	3147	WP_024906846.1	multidrug efflux RND transporter permease subunit	-
EAAEB30_RS05035	1089283..1090476	-	1194	WP_024906847.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8583.95 Da
• Isoelectric Point: 8.9008

>T144614 WP_157188699.1 NZ_CP046618:c1085202-1084984 [Enterobacter asburiae]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKVPTSVWKFVR

Nucleotide

• Length: 219 bp

>T144614 NZ_CP062203:3716046-3716264 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14481.28 Da
• Isoelectric Point: 4.8886

>AT144614 WP_008499288.1 NZ_CP046618:c1085604-1085230 [Enterobacter asburiae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT144614 NZ_CP062203:3715646-3716020 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: V3PBI9