Detailed information of TA system TA144606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/HTH_10(toxin)
• Location: 18625..19612
• Replicon: chromosome
• Accession: NZ_CP046615
• Organism: Sulfolobus acidocaldarius DSM 639

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1IU00
• Locus tag: GO597_RS00080
• Protein ID: WP_011278971.1
• Coordinates: 18625..19242 (-)
• Length: 206 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1I835
• Locus tag: GO597_RS00085
• Protein ID: WP_011278972.1
• Coordinates: 19268..19612 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GO597_RS00065 (GO597_00065)	14228..14830	+	603	WP_011278968.1	hypothetical protein	-
GO597_RS00070 (GO597_00070)	14882..16273	-	1392	WP_011278969.1	APC family permease	-
GO597_RS00075 (GO597_00075)	16424..18418	+	1995	WP_011278970.1	primary-amine oxidase	-
GO597_RS00080 (GO597_00080)	18625..19242	-	618	WP_011278971.1	helix-turn-helix domain-containing protein	Toxin
GO597_RS00085 (GO597_00085)	19268..19612	-	345	WP_011278972.1	DUF488 domain-containing protein	Antitoxin
GO597_RS00090 (GO597_00090)	19918..20706	+	789	WP_011278973.1	aldose 1-epimerase	-
GO597_RS00095 (GO597_00095)	21004..22014	+	1011	WP_147127876.1	NAD-binding protein	-
GO597_RS00100 (GO597_00100)	22602..23231	-	630	WP_011278975.1	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterioopsin activator-type, HTH domain (146-196)

Antitoxin

• Protein of unknown function DUF488 (3-108)

Sequences

Toxin

Protein

• Length: 206 a.a.
• Molecular weight: 23779.04 Da
• Isoelectric Point: 9.9689

>T144606 WP_011278971.1 NZ_CP046615:c19242-18625 [Sulfolobus acidocaldarius DSM 639]
MVFASKRPIEVTLLVDKHPCEVLDIFRKYEIKAQITNVKLRDQVTDHIAFLKINDDILKELKRRSLKTLRINENTIWIRT
NGCGVCKLLYHSDLIVEKVKVLSKATVMYKLLLPNLSALKQFLEELNSIRVEATVANVSEIDESELTDRQLEILRLAYKS
GYFDVDRKISMKELANKLGIKASTLEEILRRALKKAVKYYLDRKS

Nucleotide

• Length: 618 bp

>T144606 NZ_CP062203:1136698-1137009 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13706.92 Da
• Isoelectric Point: 9.2564

>AT144606 WP_011278972.1 NZ_CP046615:c19612-19268 [Sulfolobus acidocaldarius DSM 639]
MIKVKRVYDAPESDDGVRILIDRLWPRGVKKDLVDIWMKDIAPSDELRKWFNHEPEKWEEFKHKYMEELRSNPKVKVLIE
VIRRSENVTFLYATKSPYNNAVVLKEYIESLLTK

Nucleotide

• Length: 345 bp

>AT144606 NZ_CP062203:1137006-1137425 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGATACTAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAATAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: M1IU00

Antitoxin

• AlphaFold DB: M1I835