Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 133773..134683 Replicon plasmid pWCGKP294-1
Accession NZ_CP046613
Organism Klebsiella pneumoniae strain WCGKP294

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag GPJ73_RS27560 Protein ID WP_004144067.1
Coordinates 133773..134243 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag GPJ73_RS27565 Protein ID WP_023287184.1
Coordinates 134240..134683 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPJ73_RS27540 129999..130967 - 969 WP_074423483.1 IS5 family transposase -
GPJ73_RS27545 131272..131532 + 261 WP_017901409.1 hypothetical protein -
GPJ73_RS27550 131721..132689 - 969 WP_072096747.1 IS5 family transposase -
GPJ73_RS27555 132691..133686 - 996 WP_077254794.1 IS5 family transposase -
GPJ73_RS27560 133773..134243 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
GPJ73_RS27565 134240..134683 - 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
GPJ73_RS27570 134784..135932 - 1149 Protein_136 translesion error-prone DNA polymerase V subunit UmuC -
GPJ73_RS27575 135979..137466 - 1488 Protein_137 Mov34/MPN/PAD-1 family protein -
GPJ73_RS27580 137521..138489 - 969 WP_074424072.1 IS5 family transposase -
GPJ73_RS27585 138518..139267 - 750 Protein_139 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaSHV-12 - 1..237090 237090
- inside IScluster/Tn - - 129999..141411 11412


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T144605 WP_004144067.1 NZ_CP046613:c134243-133773 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T144605 NZ_CP062203:910077-910484 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT144605 WP_023287184.1 NZ_CP046613:c134683-134240 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT144605 NZ_CP062203:909830-910096 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References