Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5462477..5463102 Replicon chromosome
Accession NZ_CP046612
Organism Klebsiella pneumoniae strain WCGKP294

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GPJ73_RS26530 Protein ID WP_002882817.1
Coordinates 5462477..5462860 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GPJ73_RS26535 Protein ID WP_004150355.1
Coordinates 5462860..5463102 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPJ73_RS26515 5459843..5460745 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GPJ73_RS26520 5460742..5461377 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GPJ73_RS26525 5461374..5462303 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GPJ73_RS26530 5462477..5462860 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GPJ73_RS26535 5462860..5463102 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GPJ73_RS26540 5463307..5464224 + 918 WP_009484979.1 alpha/beta hydrolase -
GPJ73_RS26545 5464238..5465179 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GPJ73_RS26550 5465224..5465661 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GPJ73_RS26555 5465658..5466518 - 861 WP_004146232.1 virulence factor BrkB family protein -
GPJ73_RS26560 5466512..5467111 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T144602 WP_002882817.1 NZ_CP046612:c5462860-5462477 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T144602 NZ_CP062196:1590135-1590668 [Plesiomonas shigelloides]
GTGAGTTGGTCAGACAAATTTGTTGAGTTAGACAAGTCAAAGCATGATAGAGAGTCTTTCTATTGCGGCGAGTCAGAACT
GGATACGTTCATCCAGAAATTTGCATTGAAGCACATGAGCGCGGGTATTAGCCGGACTATGGTTTTACCCGCAAAGCCAG
TCTTACCTAATAACAAACTACCGATTAGCGCATTTTATACGATTGCACCAAGCTCGATTTGTCGTGATACATTACCTGAG
CTACTAGCAAAGAAACTACCACGCTATCCAATTCCAGTATTTTTATTAGCACAACTTTCCGTCCATAGTATGTATCAAGG
CTGTGGGCTTGGAAAAGTTGCATTGATAAAAGCTCTGCAATATTTGTGGCAAGTGAATAGCTATATACGAGCGTATGCTG
TTGTTGTTGACTGCTTAAATGAGAATGCTGAGTCATTTTATCTGAAATATGGTTTTGAATATCTTTGTGAACATAATGGT
AGAAAACGTTTGTTTTTGCCAATGAAAACGATAGAACAGTTGTTTAACTCATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT144602 WP_004150355.1 NZ_CP046612:c5463102-5462860 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT144602 NZ_CP062196:1589869-1590138 [Plesiomonas shigelloides]
ATGGCTACAGCACGTTTAGATCTTCGCATTGATGAGGAAATCAAAGCGAAAGCTGAGAAAGCATCAGCGTTGTTGGGTCT
GAAAAGCCTGACTGAGTATGTTGTTCGCTTAATGGATGAAAACTCAACAAAGGTGATTGCCGAGCATGAAAGCATCACCG
TAAGTAACGACATTTTCGATCAATTCATCGAAGCTTGCGACAAAGCAAAAGCACCTAATGAGGCATTGAAAGACGCGTTA
GCCTTTACGAAAGAGAAGGGTATCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References