Detailed information of TA system TA144600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4978044..4978560
• Replicon: chromosome
• Accession: NZ_CP046612
• Organism: Klebsiella pneumoniae strain WCGKP294

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R4YAY3
• Locus tag: GPJ73_RS24235
• Protein ID: WP_004178374.1
• Coordinates: 4978044..4978328 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A919M0P8
• Locus tag: GPJ73_RS24240
• Protein ID: WP_032434351.1
• Coordinates: 4978318..4978560 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPJ73_RS24215	4973527..4973835	-	309	WP_012737232.1	PTS sugar transporter subunit IIB	-
GPJ73_RS29805	4973920..4974093	+	174	WP_032414379.1	hypothetical protein	-
GPJ73_RS24220	4974096..4974839	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
GPJ73_RS24225	4975196..4977334	+	2139	WP_004186701.1	anaerobic ribonucleoside-triphosphate reductase	-
GPJ73_RS24230	4977576..4978040	+	465	WP_004222151.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
GPJ73_RS24235	4978044..4978328	-	285	WP_004178374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPJ73_RS24240	4978318..4978560	-	243	WP_032434351.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GPJ73_RS24245	4978638..4980548	-	1911	WP_004152270.1	BglG family transcription antiterminator	-
GPJ73_RS24250	4980571..4981725	-	1155	WP_002886900.1	lactonase family protein	-
GPJ73_RS24255	4981791..4982531	-	741	WP_002886899.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-68)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11155.97 Da
• Isoelectric Point: 10.3787

>T144600 WP_004178374.1 NZ_CP046612:c4978328-4978044 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T144600 NZ_CP062196:1576034-1576288 [Plesiomonas shigelloides]
ATGAGAATCGTATCCTTTACTGAAGCTCGTAACAGCTTGAAATCAGTACTTGATTCAGTTGTAAACGATGCAGATACAAC
CATCATTACTCGCCGTGATGCGGATGATGCAGTTGTGATGTCTCTAGATTATTACAATAGCCTGATGGAAACTGTTCACT
TGTTGCGATCCCCCGCGAACGCAGAGCATTTGAACCGTTCTATAGCTCAATTTAAAGCTGGTCGAGTCACGCAGCGAGAG
TTGAAGGATGAGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9003.47 Da
• Isoelectric Point: 6.3340

>AT144600 WP_032434351.1 NZ_CP046612:c4978560-4978318 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGGKVSLDDL

Nucleotide

• Length: 243 bp

>AT144600 NZ_CP062196:1576281-1576550 [Plesiomonas shigelloides]
ATGAGTGAAAGGCTATTGTGTTGGACTGATGAGTCTTGGGATGACTATATTTACTGGCAATCTCAGGATAAGAAAACTTT
AAAAAGAATCAATAAGTTAATTATTGATGTTAAACGCACGCCGTTTGAAGGCATCGGAAAGCCTGAAGCCTTGAGAGAAA
ACCTGTCAGGATTCTGGTCTCGCCGCATAGATGATACAAACCGTTTAGTCTATGCGGTTGATGACCTATCAATTACCATC
ATTTCTTGTCGTTATCATTATCAAGCGTAA

Structures

Toxin

• AlphaFold DB: A6THG1