Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4856100..4856910 Replicon chromosome
Accession NZ_CP046612
Organism Klebsiella pneumoniae strain WCGKP294

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag GPJ73_RS23715 Protein ID WP_004178461.1
Coordinates 4856100..4856633 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag GPJ73_RS23720 Protein ID WP_002887278.1
Coordinates 4856644..4856910 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPJ73_RS23710 4854931..4856052 + 1122 WP_004186850.1 cupin domain-containing protein -
GPJ73_RS23715 4856100..4856633 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
GPJ73_RS23720 4856644..4856910 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
GPJ73_RS23725 4857013..4858446 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
GPJ73_RS23730 4858436..4859119 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
GPJ73_RS23735 4859292..4860677 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
GPJ73_RS23740 4860695..4861039 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T144598 WP_004178461.1 NZ_CP046612:c4856633-4856100 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T144598 NZ_CP062167:2967050-2967298 [Aureimonas sp. OT7]
ATGAAAGAGATCACCTACCGACCCGCCGCAGTCAAAGCCCTTCGCAGGATGCCGGCAAATACGGCCCGGCGCATCGTGGG
GAAGATCGAAACCTATGCCGCTGACCCGTCATCGCAGGCCAATAACATCAAGAAGCTTCAGGGCCGGGATGGTATCCGTC
TTCGTCTAGGCGACTGGCGTGTTATCATGCAGGACGGTGTAGTCCTCGACGTTCTGAACATCGGCCCGCGTGGCGGGGTC
TACGACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT144598 WP_002887278.1 NZ_CP046612:c4856910-4856644 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT144598 NZ_CP062167:2967311-2967640 [Aureimonas sp. OT7]
ATGAATGAGATGGTGACCATCGAGAAGACCGAGTACGAAAGGCTTCTGAACGCTGCCGACGACCTGGCCGACATCATCGC
CTACGACCGCGCAATGGGGGATGGAGGGGAATCTATCCCTGCCGAGTTCGTCAAGCGCATGATCGACGGTGAAAGCCCGC
TCCGCGTCTATCGCGATCTGCGGGGCCTTACCCAGGCTCAGCTTTCGGAAAGCGCCGGGGTAAACCGAGTCCAGATTGCC
GATATCGAGGCAGGCCGAAAGTCCGGGTCAGTCGATACCATCAGGAAGCTTGCCACCGCCTTGTCGGTGTCGATCGATGA
TTTGGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References