Detailed information of TA system TA144582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-MazE
• Location: 24907..25502
• Replicon: plasmid unnamed2
• Accession: NZ_CP046611
• Organism: Burkholderia contaminans strain XL73

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: A0A6P2RVL2
• Locus tag: GON15_RS38840
• Protein ID: WP_039341512.1
• Coordinates: 25152..25502 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: -
• Locus tag: GON15_RS38835
• Protein ID: WP_076841588.1
• Coordinates: 24907..25158 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GON15_RS38815	20647..21930	+	1284	WP_069586065.1	hypothetical protein	-
GON15_RS38820	21927..23042	+	1116	WP_069586064.1	P-type DNA transfer ATPase VirB11	-
GON15_RS38825	23046..23186	+	141	WP_157644980.1	hypothetical protein	-
GON15_RS38830	23219..24700	+	1482	WP_069586063.1	hypothetical protein	-
GON15_RS38835	24907..25158	+	252	WP_076841588.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GON15_RS38840	25152..25502	+	351	WP_039341512.1	type II toxin-antitoxin system ChpB family toxin	Toxin
GON15_RS38845	25515..25757	+	243	WP_039341511.1	hypothetical protein	-
GON15_RS38850	25806..26159	+	354	WP_157644981.1	hypothetical protein	-
GON15_RS38855	26131..26340	+	210	WP_039341506.1	hypothetical protein	-
GON15_RS38860	26433..27086	+	654	WP_039341504.1	hypothetical protein	-
GON15_RS38865	27131..27355	+	225	WP_039341503.1	hypothetical protein	-
GON15_RS38870	27438..27788	+	351	WP_046196913.1	hypothetical protein	-
GON15_RS38875	28177..28590	+	414	WP_069586059.1	hypothetical protein	-
GON15_RS38880	28868..29332	+	465	WP_039341501.1	hypothetical protein	-
GON15_RS38885	29348..29893	+	546	WP_175035022.1	phospholipase D family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71703	71703

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-116)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12400.34 Da
• Isoelectric Point: 8.6851

>T144582 WP_039341512.1 NZ_CP046611:25152-25502 [Burkholderia contaminans]
MVRRVKFERGDIVRVSLNPTIGKEQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARYAGFAVPLSGAGTETQGVALV
NMVRTLDLEARGARKVERAPVEVVEDALARLQTILE

Nucleotide

• Length: 351 bp

>T144582 NZ_CP062160:5317977-5318462 [Escherichia coli]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8904.14 Da
• Isoelectric Point: 4.9679

>AT144582 WP_076841588.1 NZ_CP046611:24907-25158 [Burkholderia contaminans]
MELKIQKWGNSAAVRLPAVLLEQIGASAGSSLNADVRPDGVLLTPARRKYSLDELVAQCDQNAPQPADLAAWVEAKPVGH
EAW

Nucleotide

• Length: 252 bp

>AT144582 NZ_CP062160:5317708-5317986 [Escherichia coli]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A6P2RVL2