Detailed information of TA system TA144581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 136140..136789
• Replicon: plasmid unnamed1
• Accession: NZ_CP046610
• Organism: Burkholderia contaminans strain XL73

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GON15_RS38640
• Protein ID: WP_027813527.1
• Coordinates: 136140..136550 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6P2T8K5
• Locus tag: GON15_RS38645
• Protein ID: WP_027813526.1
• Coordinates: 136547..136789 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GON15_RS38605	131686..132534	-	849	WP_027813534.1	heteromeric transposase endonuclease subunit TnsA	-
GON15_RS38610	132694..132924	-	231	WP_027813533.1	hypothetical protein	-
GON15_RS38615	132935..134020	-	1086	WP_027813532.1	hypothetical protein	-
GON15_RS38620	134065..134370	-	306	WP_027813531.1	hypothetical protein	-
GON15_RS38625	134391..135044	-	654	WP_027813530.1	helix-turn-helix domain-containing protein	-
GON15_RS38630	135232..135432	-	201	WP_034176085.1	hypothetical protein	-
GON15_RS38635	135481..135828	-	348	WP_027813528.1	hypothetical protein	-
GON15_RS38640	136140..136550	-	411	WP_027813527.1	type II toxin-antitoxin system VapC family toxin	Toxin
GON15_RS38645	136547..136789	-	243	WP_027813526.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GON15_RS38650	136884..137933	-	1050	WP_027813525.1	non-homologous end-joining DNA ligase	-
GON15_RS38655	138336..139007	+	672	WP_027813524.1	AAA family ATPase	-
GON15_RS38660	139007..139972	+	966	WP_027813523.1	ParB/RepB/Spo0J family partition protein	-
GON15_RS38665	140715..141572	+	858	WP_027813522.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..152473	152473

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (12-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14869.13 Da
• Isoelectric Point: 4.8604

>T144581 WP_027813527.1 NZ_CP046610:c136550-136140 [Burkholderia contaminans]
MTLYLLDTNILSNVIRDPRGACATRIGETPPEQVCTSIIVAAELRFGVWKRGSSTLAQRVEQLLASLTVLPLQPDADRCY
GRLRAELEKQGQLIDANDMLIAAHALAVDAVLVTDNTAEFTRIAGLPVENWLRPAT

Nucleotide

• Length: 411 bp

>T144581 NZ_CP062160:5066315-5066779 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9375.64 Da
• Isoelectric Point: 4.6856

>AT144581 WP_027813526.1 NZ_CP046610:c136789-136547 [Burkholderia contaminans]
MPNHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWTPLDEIFPDIEDLPPEPVDIE

Nucleotide

• Length: 243 bp

>AT144581 NZ_CP062160:5065980-5066315 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A6P2T8K5