Detailed information of TA system TA144565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 4607486..4608107
• Replicon: chromosome
• Accession: NZ_CP046600
• Organism: Mycobacterium spongiae strain FSD4b-SM

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: F6B93_RS18680
• Protein ID: WP_211696414.1
• Coordinates: 4607486..4607923 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: -
• Locus tag: F6B93_RS18685
• Protein ID: WP_211696415.1
• Coordinates: 4607928..4608107 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6B93_RS18660 (F6B93_18655)	4603573..4604811	+	1239	WP_211696410.1	acetyl-CoA acetyltransferase	-
F6B93_RS18665 (F6B93_18660)	4604817..4606337	+	1521	WP_211696411.1	carotenoid oxygenase family protein	-
F6B93_RS18670 (F6B93_18665)	4606392..4606874	+	483	WP_211696412.1	VOC family protein	-
F6B93_RS18675 (F6B93_18670)	4606914..4607348	-	435	WP_211696413.1	hypothetical protein	-
F6B93_RS18680 (F6B93_18675)	4607486..4607923	-	438	WP_211696414.1	SRPBCC family protein	Toxin
F6B93_RS18685 (F6B93_18680)	4607928..4608107	-	180	WP_211696415.1	antitoxin	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15824.48 Da
• Isoelectric Point: 9.5656

>T144565 WP_211696414.1 NZ_CP046600:c4607923-4607486 [Mycobacterium spongiae]
MAKLSGQIDVPLLPEEAWTHASDLSRYREWLTIHRVWRSKLPETLEKGAVVESIVEVKGMPNRIKWTIVRYKPPEGMTLN
GAGVGGVKVKLIAKVTPKDDGALVSFDVHLGGPALFGPIGLIVAAALRTDIQESLQNFVTVFTPA

Nucleotide

• Length: 438 bp

>T144565 NZ_CP062160:1138096-1138410 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6754.50 Da
• Isoelectric Point: 5.0477

>AT144565 WP_211696415.1 NZ_CP046600:c4608107-4607928 [Mycobacterium spongiae]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDERTQRKYSDTIHKVQDEAKKVIDTNDQQK

Nucleotide

• Length: 180 bp

>AT144565 NZ_CP062160:1137860-1138093 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG