Detailed information of TA system TA144564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 4538484..4539067
• Replicon: chromosome
• Accession: NZ_CP046600
• Organism: Mycobacterium spongiae strain FSD4b-SM

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: F6B93_RS18465
• Protein ID: WP_246540852.1
• Coordinates: 4538714..4539067 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: F6B93_RS18460
• Protein ID: WP_211696378.1
• Coordinates: 4538484..4538681 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6B93_RS18445 (F6B93_18440)	4534152..4536485	-	2334	WP_211696375.1	ATP-dependent DNA ligase	-
F6B93_RS18450 (F6B93_18445)	4536508..4537458	+	951	WP_211696376.1	carbohydrate kinase	-
F6B93_RS18455 (F6B93_18450)	4537523..4538326	+	804	WP_211696377.1	Ku protein	-
F6B93_RS18460 (F6B93_18455)	4538484..4538681	+	198	WP_211696378.1	hypothetical protein	Antitoxin
F6B93_RS18465 (F6B93_18460)	4538714..4539067	+	354	WP_246540852.1	Fic family protein	Toxin
F6B93_RS18470 (F6B93_18465)	4539122..4539808	-	687	WP_211696380.1	maleylpyruvate isomerase family mycothiol-dependent enzyme	-
F6B93_RS18475 (F6B93_18470)	4539943..4540083	-	141	Protein_3688	IS256 family transposase	-
F6B93_RS23175	4540515..4541048	+	534	WP_246540854.1	IS110 family transposase	-
F6B93_RS23180	4540939..4541355	+	417	WP_246541188.1	IS110 family transposase	-
F6B93_RS18485 (F6B93_18480)	4542380..4543981	-	1602	WP_211696381.1	PE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4534152..4567873	33721

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-71)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12589.17 Da
• Isoelectric Point: 4.2798

>T144564 WP_246540852.1 NZ_CP046600:4538714-4539067 [Mycobacterium spongiae]
VAEYACGFQPLVRDPGLFESSLMRPATVLFGADAYPTVWDKAAALLHSLATTQSLADGNKRTGWAACWLLLGLNGHQVSA
NLDVDAAEEFVLAIAANHLTWEQIAERLPDFAESTTS

Nucleotide

• Length: 354 bp

>T144564 NZ_CP062160:c1022238-1021897 [Escherichia coli]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7110.00 Da
• Isoelectric Point: 11.0634

>AT144564 WP_211696378.1 NZ_CP046600:4538484-4538681 [Mycobacterium spongiae]
VPALNVEFSDEELAKVRSAASDAGTSMRAFARQAILDRAVNREGRVRALGQEIAQRSAELNRRLA

Nucleotide

• Length: 198 bp

>AT144564 NZ_CP062160:1022233-1022309 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT