Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 3027081..3027616 Replicon chromosome
Accession NZ_CP046600
Organism Mycobacterium spongiae strain FSD4b-SM

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag F6B93_RS12340 Protein ID WP_211695358.1
Coordinates 3027284..3027616 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag F6B93_RS12335 Protein ID WP_211695357.1
Coordinates 3027081..3027287 (+) Length 69 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6B93_RS12315 (F6B93_12310) 3022966..3023088 - 123 WP_068914732.1 type B 50S ribosomal protein L36 -
F6B93_RS12320 (F6B93_12315) 3023307..3024167 + 861 WP_211695354.1 mycofactocin-coupled SDR family oxidoreductase -
F6B93_RS12325 (F6B93_12320) 3024489..3025406 + 918 WP_211695355.1 hypothetical protein -
F6B93_RS12330 (F6B93_12325) 3025541..3026386 + 846 WP_211695356.1 TIM barrel protein -
F6B93_RS12335 (F6B93_12330) 3027081..3027287 + 207 WP_211695357.1 DUF3018 family protein Antitoxin
F6B93_RS12340 (F6B93_12335) 3027284..3027616 + 333 WP_211695358.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
F6B93_RS12345 (F6B93_12340) 3027809..3029158 - 1350 WP_211695359.1 multidrug effflux MFS transporter -
F6B93_RS12350 (F6B93_12345) 3029229..3029687 + 459 WP_211695360.1 MarR family winged helix-turn-helix transcriptional regulator -
F6B93_RS12355 (F6B93_12350) 3030289..3030528 - 240 WP_211695361.1 hypothetical protein -
F6B93_RS12360 (F6B93_12355) 3030740..3031039 - 300 WP_211695362.1 type II toxin-antitoxin system RelE/ParE family toxin -
F6B93_RS12365 (F6B93_12360) 3031036..3031287 - 252 WP_211695363.1 type II toxin-antitoxin system ParD family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-105)

Antitoxin

Antitoxin MazE-like

(4-66)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12065.92 Da        Isoelectric Point: 5.8334

>T144562 WP_211695358.1 NZ_CP046600:3027284-3027616 [Mycobacterium spongiae]
VNRGEIWTVAGDVYAMKPRPAVIVQDDLFDVTKSVTVAPITSTLLDAPLMRIRITGGDGRLSGLDHDSDVMIDKLTTVRR
SNVQDRVGRLTAEQVVEVDRAMMAFLGLAR

Download         Length: 333 bp

>T144562 NZ_CP062160:791959-792132 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 69 a.a.        Molecular weight: 7995.91 Da        Isoelectric Point: 6.4683

>AT144562 WP_211695357.1 NZ_CP046600:3027081-3027287 [Mycobacterium spongiae]
MAVRERVAEYRRRMRERGLRPLQVWVPDVRTESFAAEAHRQASLVARADESSDHQDFIKAVSTPWDEE

Download         Length: 207 bp

>AT144562 NZ_CP062160:791635-791931 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References