Detailed information of TA system TA144554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1367344..1368260
• Replicon: chromosome
• Accession: NZ_CP046592
• Organism: Bacillus subtilis strain TR21

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: GO005_RS07195
• Protein ID: WP_003244695.1
• Coordinates: 1367514..1368260 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: GO005_RS07190
• Protein ID: WP_003232646.1
• Coordinates: 1367344..1367514 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GO005_RS07155	1364207..1364536	+	330	WP_003232660.1	phage-like element PBSX protein XkdW	-
GO005_RS07160	1364533..1364697	+	165	WP_003232658.1	XkdX family protein	-
GO005_RS07165	1364741..1365580	+	840	WP_032721388.1	phage-like element PBSX protein XepA	-
GO005_RS07170	1365633..1365902	+	270	WP_003232655.1	hemolysin XhlA family protein	-
GO005_RS07175	1365915..1366178	+	264	WP_003232653.1	phage holin	-
GO005_RS07180	1366191..1367084	+	894	WP_003245230.1	N-acetylmuramoyl-L-alanine amidase	-
GO005_RS07185	1367121..1367258	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
GO005_RS07190	1367344..1367514	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
GO005_RS07195	1367514..1368260	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
GO005_RS07200	1368370..1369371	-	1002	WP_003232642.1	inorganic phosphate transporter	-
GO005_RS07205	1369384..1370001	-	618	WP_003218470.1	DUF47 domain-containing protein	-
GO005_RS07210	1370277..1371593	-	1317	WP_003244819.1	serine/threonine exchanger	-
GO005_RS07215	1371982..1372932	+	951	WP_003245086.1	ring-cleaving dioxygenase	-
GO005_RS07220	1373033..1373179	+	147	WP_003244977.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T144554 WP_003244695.1 NZ_CP046592:c1368260-1367514 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T144554 NZ_CP062156:3513704-3514132 [Stenotrophomonas sp. CW117]
ATGCGTGCGTTGCTTGACGTGAACGTGCTGATCGCACTGCACGACAGCGACCACGTCCACCATCACCCTGCCTCGGCCTG
GCTGCGCACGCACATCGCGCATGGCTGGGCCAGTTGTCCACTTACCCAGAACGGTTGCCTGCGCATCATGTCCCAGCCCG
GATACGCCAAGCCGCAACCGCTGGCCAGCCTGGTGAGCATGCTGGCGCGTTCCACGTCCACGCGCTTCCATGCGTTCTGG
CCGGACGCCATCAGCGTGCTGGATCGCACGCATTTCCATCATGGCTACATCCACAGTCCGCGACAGTTGACCTATCTGTA
CCTGCTCGCGCTCGCGGTGCACCACGGCGGGCGGCTGGTGACGTTCGACCGGCACATTGCGCTGAGTGCGGTAGCGGGCG
CGCGGCCCGAGCACCTGGTGCAGTTGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT144554 WP_003232646.1 NZ_CP046592:c1367514-1367344 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT144554 NZ_CP062156:3513432-3513704 [Stenotrophomonas sp. CW117]
ATCAATCATCAGGGAGAGCCGCATGCGCCCTCCCTCGACATTGCCGACGACGTGCTGTTTGCCGCCAAGGAGCTGGCACG
CCGCGAGAAGAAGCCACTTGGCCAGGTGATCAGCGAGTTGGCGCGGCGGGCGTTCGCCCAGTCCGATGCGCCCTGGGACA
CGACCGGCGTGGCCGAACCCTTGGCCGATTACGGCATTCATCCGCTGCCACCACGCGGCGTGGTGGTCAGCAATGAACTC
ATCGATCGCCTGCGCGACGAAGAGGGCATCTGA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• AlphaFold DB: G4EXD8