Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 572853..573489 Replicon chromosome
Accession NZ_CP046592
Organism Bacillus subtilis strain TR21

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GO005_RS02990 Protein ID WP_003156187.1
Coordinates 573139..573489 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag GO005_RS02985 Protein ID WP_003225183.1
Coordinates 572853..573134 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GO005_RS02965 569212..569811 - 600 WP_032722928.1 rhomboid family intramembrane serine protease -
GO005_RS02970 569906..570271 + 366 WP_015252768.1 holo-ACP synthase -
GO005_RS02975 570437..571453 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
GO005_RS02980 571568..572737 + 1170 WP_032722929.1 alanine racemase -
GO005_RS02985 572853..573134 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GO005_RS02990 573139..573489 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GO005_RS02995 573604..574428 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
GO005_RS03000 574433..574798 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
GO005_RS03005 574802..575203 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
GO005_RS03010 575215..576222 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
GO005_RS03015 576284..576613 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
GO005_RS03020 576610..577092 + 483 WP_003234299.1 anti-sigma B factor RsbW -
GO005_RS03025 577058..577846 + 789 WP_029727187.1 RNA polymerase sigma factor SigB -
GO005_RS03030 577846..578445 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T144553 WP_003156187.1 NZ_CP046592:573139-573489 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T144553 NZ_CP062152:c812650-811325 [Vibrio parahaemolyticus]
ATGGTGAAGCAATTACGCGTGTTAATGAATGGTGAGTCAGTAGGGGTGTTCACTAAAGAACCTAGTGGTGCTCATAGCTT
TCAATACGGAGAAGACTGGTTAAACTCTAGTAAATCTCGCCCGATATCATTATCAATCCCCCTTCGCCGTGAGCCGTACA
GGGGTGATATAGTGATTAACTTTTTCGACAACCTGCTTCCAGATAACCCTCAGGTTCGAGACCGAATCGTTGCTCGATAC
GGTGCCAAAACAAAACAACCATTCGATCTTTTGGAAAAGATAGGGCGCGATAGCGTTGGGGCAATCACTCTTATTCCTGA
AGGCGAGCCGATACCAGAATTTAAGGGAATAAGAGCTACTGAGCTTTCCCTTATAAAGCTTGAACAAGTTCTTAATTCCT
ATGCAGACAATATCCCTTTAGGAATGGTTGATGGAAATGATGATTTCAGGATCTCCATTGCTGGCGCTCAAGAGAAAACT
GCCTTGTTGAAGTTAGATGGGCGTTGGTATATACCGCAAGGTGCAACTCCAACCACACACATTATAAAACTACCTATTGG
AGAAATACGGACGCAGGACAGCGTTTTAGACCTAACAACCAGCGTTGATAATGAACTCGTGTGTATGAGACTTTCTGAAG
CGTTTGGACTTCCTACAGCCTCTTGTGACGTTATTAGGGCTGGTGGAGTAAGGGCGCTTGCCGTTGAGCGTTTCGATAGA
CGATTCTCACAACTTAAGGATTGGATTGTACGCTTGCCTCAAGAAGACTTTTGCCAGGTGTCTGGTATGCCATCAGCAAG
CAAGTATGAATCAGATGGTGGTATCGGTATTGAAGGAATTATGGATCACCTTCTTGGCTCAGAGGTTCCTCAGGAAGACA
GAAGAACCTTTATGCGTTCTCAAGTTCTGTTCTATTTACTTGCTGCAATAGATGGTCACTCAAAAAACTTTTCGTTATTC
ATAGAGCCTCATGGTAAGTACCGATTAACGCCTTTGTACGACATTATGTCAGCCTACCCTCTTATGGGTGGTAGAGGGCT
TCATGAAAGAAAAATCAAGCTGGCAATGGGGCTTAAGGCAAGTAGCGGATACAAGCGTAAGTGGCATCAGATACAGCCTA
GACATTTTATCGGTACAGCGAGGCTGGTGAAGTATTCATCGGACTCAATGAGAGATATTCTGGATGAGTTTATAACTGAT
TGTGGACAGGTTATCGATCTGGTTGGGAACGATTTGCCAGATGATATCAATGAGAATGTTCGGGATAAAATTTTTGAGGG
ATTAACAAAATCAGTGAAAAAACTGAGTCTTGGTCTGAATGAGTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT144553 WP_003225183.1 NZ_CP046592:572853-573134 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT144553 NZ_CP062152:c812901-812644 [Vibrio parahaemolyticus]
ATGTTGATAACATCATCCAGGCAGTTAGCAATACATCTTCGTGATGAAAGAAAAAGCAGGAAACTATCTCAAGCAAAAGT
TGCCGATCCAGTAGGGATAAAACAAGACACGGTTTCTAAGTTTGAGGTTAATCCTGAAACCACCAGCATTCAGACGCTAT
TTCGAATCTTATCATCGTTAAATTTGGAAATGCATATTGTGCCGAAAGGTCAAAGCATCGAACCAAAAACTGATAATGAG
AGTGATGAGACATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References