Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2163987..2164623 Replicon chromosome
Accession NZ_CP046591
Organism Bacillus subtilis strain R31

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GO004_RS11425 Protein ID WP_003156187.1
Coordinates 2163987..2164337 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag GO004_RS11430 Protein ID WP_003225183.1
Coordinates 2164342..2164623 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GO004_RS11385 2159030..2159629 - 600 WP_003246608.1 phosphoserine phosphatase RsbX -
GO004_RS11390 2159629..2160417 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
GO004_RS11395 2160383..2160865 - 483 WP_038427419.1 anti-sigma B factor RsbW -
GO004_RS11400 2160862..2161191 - 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
GO004_RS11405 2161253..2162260 - 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
GO004_RS11410 2162272..2162673 - 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
GO004_RS11415 2162677..2163042 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
GO004_RS11420 2163047..2163871 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
GO004_RS11425 2163987..2164337 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GO004_RS11430 2164342..2164623 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GO004_RS11435 2164739..2165908 - 1170 WP_195727637.1 alanine racemase -
GO004_RS11440 2166022..2167038 - 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
GO004_RS11445 2167204..2167569 - 366 WP_014475874.1 holo-ACP synthase -
GO004_RS11450 2167664..2168263 + 600 WP_195727638.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T144552 WP_003156187.1 NZ_CP046591:c2164337-2163987 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T144552 NZ_CP062150:c830648-830370 [Vibrio parahaemolyticus]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAAATCTTCGAGTTTCTCTATGACTTCAACCCTGATGCGGC
AGAGAAAACTGACAACCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATGGCA
TCCGCGGACGATTGCTCATTATTCCTGAGATTTCGATGATTGTCTCTTACTGGGTCGAGGGCGATATCATCCGAGTTATG
CGTGTACTCCACCAGAAACAAAAATTCCCTACGGATTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT144552 WP_003225183.1 NZ_CP046591:c2164623-2164342 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT144552 NZ_CP062150:c830929-830645 [Vibrio parahaemolyticus]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAGAGCCAAGGTCGCAC
TCTGAGTGACGCATGCCGTGAACTTACTGAGCAACTTGCTGAACAACAAAGAAAAACATTATCTCACGATGCATGGCTAA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAGTCCGTTTTCGTTGAGCACCAAACTGCTAAATCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGCAATCGAGGTAAGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References