Detailed information of TA system TA144551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1360612..1361528
• Replicon: chromosome
• Accession: NZ_CP046591
• Organism: Bacillus subtilis strain R31

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: GO004_RS07185
• Protein ID: WP_003244695.1
• Coordinates: 1360612..1361358 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: GO004_RS07190
• Protein ID: WP_003232646.1
• Coordinates: 1361358..1361528 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GO004_RS07165	1355940..1356890	-	951	WP_015715745.1	ring-cleaving dioxygenase	-
GO004_RS07170	1357279..1358595	+	1317	WP_015252262.1	serine/threonine exchanger	-
GO004_RS07175	1358871..1359488	+	618	WP_003218470.1	DUF47 domain-containing protein	-
GO004_RS07180	1359501..1360502	+	1002	WP_014479569.1	inorganic phosphate transporter	-
GO004_RS07185	1360612..1361358	+	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
GO004_RS07190	1361358..1361528	+	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
GO004_RS07195	1361614..1361751	+	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
GO004_RS07200	1361789..1362682	-	894	WP_195727878.1	N-acetylmuramoyl-L-alanine amidase	-
GO004_RS07205	1362695..1362958	-	264	WP_014479566.1	phage holin	-
GO004_RS07210	1362971..1363240	-	270	WP_015252265.1	hemolysin XhlA family protein	-
GO004_RS07215	1363293..1364132	-	840	WP_088467634.1	phage-like element PBSX protein XepA	-
GO004_RS07220	1364176..1364340	-	165	WP_015715740.1	XkdX family protein	-
GO004_RS07225	1364337..1364666	-	330	WP_003232660.1	phage-like element PBSX protein XkdW	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T144551 WP_003244695.1 NZ_CP046591:1360612-1361358 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T144551 NZ_CP062147:c3257036-3256707 [Komagataeibacter hansenii]
ATGTCAGCATGGGTGCCAGATTGCGGAGACATCGTCTGGCTGGAGTTTGATCCTCAGGCAGGGCGTGAACAATCTGGGCA
TCGCCCGGCTGTGGTGCTTTCGCCAGTTAGTTACAACAAAAAATCTGGTATGATTGTTTGTTGCCCGACAACGACAAAAA
TCAAAGGGTATCCCTTTGAAGTGCCTTTGACAGGAGAGCCGACAAGCGTGGTTCTCTCTGATCAGGTTCGCAGTCTTGAT
TGGCGCGCCAGACGAGCAAAGCATAAAGGTCGGGTCTCCGAAAGAGAACTGGAAGCGGTGCGCGAACGAACAAGACTACT
CGTCGGGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT144551 WP_003232646.1 NZ_CP046591:1361358-1361528 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT144551 NZ_CP062147:c3257279-3257037 [Komagataeibacter hansenii]
ATGCATGGGACCGTTCGCAAATGGGGGAACAGCGCTGCCATCCGTCTACCAGCCAATATCCTTGAAGCGGTTCACCTACA
GATCAATCAGGCTGTTGATGTCCGGGAAGAAGATGGGCGTATCATCATTCAACCGGTTCACCCTGCTGTCCTTTCGCTGG
ACACCCTGACTGCCGCCATCACGGATGATAACCGTCACGGTGAAGTGAGTTTTGGGCCTGAAATTGGCGGAGAGATCTGG
TAA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8