Detailed information of TA system TA144541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3700442..3701072
• Replicon: chromosome
• Accession: NZ_CP046582
• Organism: Erwinia sp. E602

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: GKQ23_RS18540
• Protein ID: WP_056240482.1
• Coordinates: 3700854..3701072 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: GKQ23_RS18535
• Protein ID: WP_056240478.1
• Coordinates: 3700442..3700822 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ23_RS18505 (GKQ23_18455)	3696562..3696822	+	261	WP_212409125.1	type B 50S ribosomal protein L31	-
GKQ23_RS18510 (GKQ23_18460)	3696833..3696958	+	126	WP_056240468.1	type B 50S ribosomal protein L36	-
GKQ23_RS18515 (GKQ23_18465)	3697107..3697985	-	879	WP_056240471.1	metal ABC transporter substrate-binding protein	-
GKQ23_RS18520 (GKQ23_18470)	3697988..3698851	-	864	WP_056240473.1	metal ABC transporter permease	-
GKQ23_RS18525 (GKQ23_18475)	3698848..3699537	-	690	WP_249168430.1	ATP-binding cassette domain-containing protein	-
GKQ23_RS24100	3699534..3699662	-	129	WP_255354469.1	hypothetical protein	-
GKQ23_RS18530 (GKQ23_18480)	3699953..3700306	+	354	WP_056240476.1	hypothetical protein	-
GKQ23_RS18535 (GKQ23_18485)	3700442..3700822	+	381	WP_056240478.1	Hha toxicity modulator TomB	Antitoxin
GKQ23_RS18540 (GKQ23_18490)	3700854..3701072	+	219	WP_056240482.1	HHA domain-containing protein	Toxin
GKQ23_RS18550 (GKQ23_18500)	3701434..3701748	+	315	WP_249168431.1	MGMT family protein	-
GKQ23_RS18555 (GKQ23_18505)	3701797..3702354	-	558	WP_212409127.1	YbaY family lipoprotein	-
GKQ23_RS18560 (GKQ23_18510)	3702579..3703442	+	864	WP_056240499.1	acyl-CoA thioesterase II	-
GKQ23_RS18565 (GKQ23_18515)	3703535..3704821	-	1287	WP_056240580.1	ammonium transporter AmtB	-
GKQ23_RS18570 (GKQ23_18520)	3704856..3705194	-	339	WP_056240502.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8673.15 Da
• Isoelectric Point: 8.9007

>T144541 WP_056240482.1 NZ_CP046582:3700854-3701072 [Erwinia sp. E602]
MTDKMLTKTDYLMRLRRCRSIDTLERVIEKNKYELTDDELAVFYSAADHRLAELTMNKLYDKIPVAVWKFVR

Nucleotide

• Length: 219 bp

>T144541 NZ_CP062146:c759869-759444 [Proteus mirabilis]
ATGATTAAATACCTATTAGATACAAATATTGTTATATTCACTATTAAACGCAAGCCTGAGTTTTTATTACCAAAATTCAA
CCAACATGCTGAACAATTAGCTATTTCTACAATTACACTCGCTGAGCTCATTTTTGGTGCTGAAAAAAGTGTAAATCCAG
CCAAAAACTTAGCTACGGTTAACGACTTTGTTTCTCGACTGACTGTTTTATCTTACGATGACTTAGCAGCTTTTCATTAT
GGAGACATTAGAGCAACACTAGAAAAACAGGGAAAACGCATTGGTGATAACGACTTACATATAGCAGCACATGCTAGAAG
TAAGGGGCTAATAGTCGTTACCAATAATACTCGAGAATTTGAAAGAGTTGATGGTTTAAGAATTGAGGATTGGACCGTTA
TTAACTCAGACCCCCAAACTGATTGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14510.24 Da
• Isoelectric Point: 4.7216

>AT144541 WP_056240478.1 NZ_CP046582:3700442-3700822 [Erwinia sp. E602]
MDEYSPKRHDIAQLKFLCENLYDESMATLGDSHHGWVNDPTSATNLQLNELIEHIAAFTVNYKIKHVEEHLLISQIDEFL
DDTFMLFSNYGINVQDLQRWQKSAKRLLSIFAEDAAAGQIQPSHSF

Nucleotide

• Length: 381 bp

>AT144541 NZ_CP062146:c760104-759862 [Proteus mirabilis]
ATGATCCAGTATGGCAAAGTATTTATGAGCAATAGAACACAAAATGTTCGATTACCCGCTGAGACTCGATTTCCGGATGA
TGTAAAAGAAGTTTTTGTTCGCGTCAAAGGCAAAGAGAGAATAATCACACCTATACAAAATGCATGGGATAGCTTTTTCT
TATCAGAAAAGTCTGTAACAGATGATTTCTTATCTGAAAGGCCTGATCAAGCCACAAGTGAAAGAGAATCCTTTGATGAT
TAA