Detailed information of TA system TA144528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 979801..980831
• Replicon: chromosome
• Accession: NZ_CP046582
• Organism: Erwinia sp. E602

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: GKQ23_RS05820
• Protein ID: WP_212410014.1
• Coordinates: 979801..980376 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: GKQ23_RS05825
• Protein ID: WP_072166260.1
• Coordinates: 980373..980831 (-)
• Length: 153 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ23_RS05790 (GKQ23_05775)	975167..975940	-	774	WP_056232099.1	amino acid ABC transporter substrate-binding protein	-
GKQ23_RS05795 (GKQ23_05780)	975975..976742	-	768	WP_212410010.1	amino acid ABC transporter ATP-binding protein	-
GKQ23_RS05800 (GKQ23_05785)	976735..977418	-	684	WP_056232103.1	amino acid ABC transporter permease	-
GKQ23_RS05805 (GKQ23_05790)	977744..978307	-	564	WP_249168478.1	TetR/AcrR family transcriptional regulator	-
GKQ23_RS05810 (GKQ23_05795)	978445..978984	+	540	WP_212410012.1	HPP family protein	-
GKQ23_RS05815 (GKQ23_05800)	979040..979645	-	606	WP_212410013.1	inorganic diphosphatase	-
GKQ23_RS05820 (GKQ23_05805)	979801..980376	-	576	WP_212410014.1	PIN domain-containing protein	Toxin
GKQ23_RS05825 (GKQ23_05810)	980373..980831	-	459	WP_072166260.1	excisionase family DNA-binding protein	Antitoxin
GKQ23_RS05830 (GKQ23_05815)	980916..981275	-	360	WP_048917133.1	6-carboxytetrahydropterin synthase QueD	-
GKQ23_RS05835 (GKQ23_05820)	981564..983354	+	1791	WP_101505299.1	NADPH-dependent assimilatory sulfite reductase flavoprotein subunit	-
GKQ23_RS05840 (GKQ23_05825)	983354..985081	+	1728	WP_212410015.1	assimilatory sulfite reductase (NADPH) hemoprotein subunit	-
GKQ23_RS05845 (GKQ23_05830)	985083..985814	+	732	WP_101505298.1	phosphoadenylyl-sulfate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-115)

Antitoxin

• SinI-like, DNA-binding domain (81-125)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21627.89 Da
• Isoelectric Point: 6.3385

>T144528 WP_212410014.1 NZ_CP046582:c980376-979801 [Erwinia sp. E602]
MSAGYAVVLDACVLYPAPLRSFLMYLAAGGQFRAHWTEDIHEEWIRNLLLNRKELARPQLERVRALMDRHAPDARVTGYR
ELIDSITGLPDKDDRHVVAAAIVAQAAEIVTFNLKDFPDEVLTRWNLRAIHPDSFITSLSDLDITLVLEAARRQRASLKS
PPFSADEFLDCLLRQQLPETVSRLRPLSGLL

Nucleotide

• Length: 576 bp

>T144528 NZ_CP062122:c4675610-4675263 [Pseudomonas sp. ADPe]
ATGTCCAAGGTCGTCATCCGTCTCACCGATACCGCAGAACAAAGCATCGAAGACCAAATACACCACTTGGCTCAATTCCA
AGGTTCGCAGGCTGCACTCCAGTCGGTGCTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCTCTTACACCCCAAGGTT
ACCCAATCAGCCAGCAGGCAAGCCTTTTGGGGGTGTTGAACTATCGCGAACTCAATACCGGCCCTTATCGCGTCTTTTAC
GAACTACACGAAGAGCAAGGCGAGGTCGCGGTTATCCTGGTCTTACGCCAGAAGCAGAGTGTGGAACAGCAATTGATTCG
CTACTGCTTGGTGGGGCCTATCGGGTGA

Antitoxin

Protein

• Length: 153 a.a.
• Molecular weight: 17015.49 Da
• Isoelectric Point: 7.2795

>AT144528 WP_072166260.1 NZ_CP046582:c980831-980373 [Erwinia sp. E602]
MTTLQNKLPSQHEAELAKLCSRELSAILETRAETQQVSVTDKRGQVHNVRIPVNALRLLVDVLTELGEGNAVRLVPVGAE
MTTQEAADMLNISRPTLIRLLDSAELPYHRTGNRRKIRFSDVLAYRDRLKNARLDALDQLGAIDQQLGMGYE

Nucleotide

• Length: 459 bp

>AT144528 NZ_CP062122:c4675871-4675620 [Pseudomonas sp. ADPe]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGCCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACACAGAA
CGGTGTTCCTGCCTACGTGATTGAGTCATATGCAGAACGAAAGCAACGTGACGAAGCAATTGCGTTGGTGAAGCTGCTTG
CGATTGGCTCTCGTCAATACGCAGAAGGCAAACATCGCTCTGTAGATGATCTGAAAGCTCGTCTTTCCAGGAGGTTCGAT
CAACCAGAATAA