Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 120786..121351 Replicon plasmid pRWAY01
Accession NZ_CP046573
Organism Rhodococcus sp. WAY2

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag GFS60_RS30560 Protein ID WP_159927854.1
Coordinates 120995..121351 (+) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag GFS60_RS30555 Protein ID WP_159927852.1
Coordinates 120786..120998 (+) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFS60_RS30530 116309..117193 + 885 WP_159927850.1 crotonase/enoyl-CoA hydratase family protein -
GFS60_RS30535 117452..117703 + 252 Protein_117 WhiB family transcriptional regulator -
GFS60_RS30540 117848..118090 + 243 Protein_118 phosphate transport system regulatory protein PhoU -
GFS60_RS30545 118475..119706 + 1232 WP_159927925.1 IS3 family transposase -
GFS60_RS30550 119767..120303 + 537 WP_159929025.1 PAS and ANTAR domain-containing protein -
GFS60_RS30555 120786..120998 + 213 WP_159927852.1 ribbon-helix-helix domain-containing protein Antitoxin
GFS60_RS30560 120995..121351 + 357 WP_159927854.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GFS60_RS30565 121601..121870 - 270 WP_159927856.1 hypothetical protein -
GFS60_RS30570 121872..122934 - 1063 Protein_124 IS110 family transposase -
GFS60_RS30580 124394..124975 - 582 WP_159927858.1 hypothetical protein -
GFS60_RS30585 125245..125709 - 465 WP_159927860.1 DUF4383 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..991117 991117
- inside IScluster/Tn - - 118466..124178 5712


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-114)

Antitoxin

Ribbon-helix-helix protein, CopG

(4-39)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12939.72 Da        Isoelectric Point: 8.9647

>T144517 WP_159927854.1 NZ_CP046573:120995-121351 [Rhodococcus sp. WAY2]
MIRRGHVHWVDLDPAQGSEAAKRRPAVVVSNDRANATAERLGQGVVTVVPLTSNTAHVFAFQVLVDPDESGMPRESKAQA
EQVRSVSVRRLDLEPVGKLSTRTLAALEEALRLHLDLR

Download         Length: 357 bp

>T144517 NZ_CP062121:2733707-2734576 [Variovorax sp. 38R]
ATGGGCACCATCGACATCTCCGCGCAGACCCTGCCGGCTTCCGAGCTGGCGCGGCTCGACGCACGCTTTCCGGCCCGCGA
CGTCGCGGTCGGCGGCGGCGCGGTGGTGTCGGTGCGCGAATGCGGACAGGGCCCGGTGATCGTGTGCCTGCATGGCATCG
GCTCGGGCGCGGCCTCGTGGCTCGACACCGCGGCGCTGCTGGCGCCGCAGGCCTGCCTCATCGCGTGGGACGCACCGGGC
TACGGCCGCTCGACGCCGCTGGCCTCGGCTGCGCCGACCGCCGCCGACTACGCCGCACGCTTGGGCGAATTGCTCGACGC
GCTCTCGATCCGCTCCTGCGTGCTCGTCGGCCATTCGCTGGGTGCCATCACCGCCGCATCGGCTGCGCGCAGCGAGTCGA
GCGCCAGTGCGCGCATCCGCCGCCTCGTGCTCGTGAGCCCCGCCATCGGCTACGGCGCCCCCGAGCGCATCGACGCCCGC
GCCAAGGTGCGCGCCGAACGCCTGGCCACGCTCGACGACCTCGGCATTGCCGGCATGGCCGCCCAACGCGCGGGCCGGCT
CGTGTCCGACGGTGCCAGCGAGCACGCCCGCCAGTGGGTGCGCTGGAACATGGCGCGCCTGAACGACCACGGCTACCGCC
AGGCCGTCGAGCTGCTGTGCAACGCCGACCTGCTGGCCGACCTGCCGCCCCCGATGCCCGTGCGCGTGGCCTGCGGTGCG
CTCGACGTCGTCACGCCGCCGGCCGCCTGCGAAGAAGTCGCGCGGCGCTGCGGCGTGCCCCTCGAATTGGTGGACGATGC
GGGTCACGCCGGCTATGTGGAACAACCGCAGGCCGTCGCCACCTTGCTGCGCGAATCGCTCGCCGGCTGA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 7516.23 Da        Isoelectric Point: 4.0424

>AT144517 WP_159927852.1 NZ_CP046573:120786-120998 [Rhodococcus sp. WAY2]
MTTRFTISLPDEDARVLDAYVRDHGLGGRSAGIRAAVRMLGAVGLERDYAAAFVEGGVDDDWDVTSADDE

Download         Length: 213 bp

>AT144517 NZ_CP062121:2734613-2735446 [Variovorax sp. 38R]
ATGGCCACCGACAACGAAGTGATGGAAGAGGGCGCAGACCGCTACAACGTGCCCGCGCTGGAGCGCGGCCTGCGCGTGCT
GTGCGAGTTCAGCCGCGAGAGCCGCACGCTCTCCGCGCCTGAGCTGGCACGCCGCTTCGACCTGCCGCGCTCGACCGTCT
TCCGCCTGCTCACCACGCTGGAGAACATGGGTTTCCTCGAGCGCGCCGAGGGCGGCCGCGACTACCGCCTGGGCCTGGCC
GTGCTGCGCCTGGGCTTCGAATACCTGGCCTCGCTGGAACTCACGCAACTGGGCACGCCGCTGCTCAACCGCCTGTGCGA
CGAGCTGCGCACGCCCTGCAACCTGGTGGTGCGCGATGGCCGCTCGATCGTCTACGTTGCCAAGGTGGCACCGGCCACGC
CGTTTGCCAGCTCGGTCACGGTGGGCACGCGCCTGCCGGCCCACGCCACGGTGCTGGGCCGCATCCTGCTCGAAGACCTC
ACGCTGCCGCAGCTGCGCGCGCTGTACCCCGAAGACAAGCTCGAGACCTTCTCGCCGAGCACGCCCAAGACCGTGAACGA
ACTGTTCGACATGGTGCAGGCCGACCGCCAGCGCGGCTATGTGCTGGGCGAGGGCTTCTTCGAATCGAACATCTCGACCA
TCGCCGCACCGGTGCGCGACCACAGCGGCCACATCGTGGCGGCGCTGGGCGCCACCATCACCTCGGGCCACATCGACGAG
AACCGCATGGACGAGATGGTGCTGCGCGTGCGCGGCACGGCCGACGAGATCTCGGGCCTGCTCAATTACTCGCCTGCACG
CACCGCCAAGGTGGTTCCGCTGCGCAGCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References