Detailed information of TA system TA144515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 4336029..4336570
• Replicon: chromosome
• Accession: NZ_CP046572
• Organism: Rhodococcus sp. WAY2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GFS60_RS19900
• Protein ID: WP_159923892.1
• Coordinates: 4336271..4336570 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GFS60_RS19895
• Protein ID: WP_159923890.1
• Coordinates: 4336029..4336271 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFS60_RS19875	4331142..4332542	-	1401	WP_159923885.1	3-isopropylmalate dehydratase large subunit	-
GFS60_RS19880	4332603..4333874	-	1272	WP_159927365.1	2-hydroxycarboxylate transporter family protein	-
GFS60_RS19885	4334267..4334632	+	366	WP_159923886.1	metalloregulator ArsR/SmtB family transcription factor	-
GFS60_RS19890	4334641..4335837	+	1197	WP_201453404.1	CoA transferase	-
GFS60_RS19895	4336029..4336271	+	243	WP_159923890.1	antitoxin	Antitoxin
GFS60_RS19900	4336271..4336570	+	300	WP_159923892.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GFS60_RS19905	4336926..4337089	-	164	Protein_3935	IS110 family transposase	-
GFS60_RS19910	4337116..4337511	-	396	WP_159923894.1	DUF5313 family protein	-
GFS60_RS19915	4337508..4337978	-	471	WP_159923896.1	MarR family transcriptional regulator	-
GFS60_RS19920	4338090..4338515	-	426	WP_109335379.1	hypothetical protein	-
GFS60_RS19925	4338875..4339939	-	1065	WP_159923898.1	methyltransferase domain-containing protein	-
GFS60_RS19930	4339977..4340789	-	813	WP_159923900.1	energy-coupling factor ABC transporter ATP-binding protein	-
GFS60_RS19935	4340786..4341550	-	765	WP_159923902.1	cobalt ECF transporter T component CbiQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-95)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10526.21 Da
• Isoelectric Point: 7.2793

>T144515 WP_159923892.1 NZ_CP046572:4336271-4336570 [Rhodococcus sp. WAY2]
MRPIHAAKLDKTRPVLVLTRELVRPHLSRVTVAPITGTVRGLSTEVPVGPANGLEKDSVVSCDNIVTIPVSALGHGLGFF
FPSQEAALSEAIRAAFDLE

Nucleotide

• Length: 300 bp

>T144515 NZ_CP062120:c133988-133707 [Aminobacter sp. SR38]
ATGCGGCAGATTGAGCGCACCGGCCAGTTCAAGCGCGACTACAAGCGCGAGGCGAAAGGGCAGCACCGGGCCACGCTCGA
CGCCGACCTGGTGCCCGTCCTGGTCGCGCTCGCCGACGACCAGCCGCTGGAACCTCGGCACCGCGACCACGCGCTGACCG
GGGACTGGAAGGATCACCGGGATTGCCACGTCAAGCCCGACCTGGTGCTGATCTACCAGAAGCCCGACGCCGACACGCTG
CGCCTGGTGCGCCTCGGCTCTCACTCCGAACTCGGCTTGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8918.97 Da
• Isoelectric Point: 4.8668

>AT144515 WP_159923890.1 NZ_CP046572:4336029-4336271 [Rhodococcus sp. WAY2]
MSTQIAVRLPDEIVAFVDGEVREHRAPSRAALVLRALERERRRQIAARDAEILSRARGEDDPDGLDDLARHAAGLFSDLD

Nucleotide

• Length: 243 bp

>AT144515 NZ_CP062120:c134238-133975 [Aminobacter sp. SR38]
ATGGCTGCAAACCAGCTCGTACAAACGCGCATCGACGGCGCGATCAAGGAAGAAGCGGCGGCCGTCCTGGCCGCGATGGG
CCTTACCGTGTCCGACGCGGTGCGGCTGCTGCTCACGAAGGTGGCCCAGGACAAGGCGCTGCCCTTCGAGCCGCTGATTC
CGAACGCCACCACCATCGAGGCGATGAAGGAAGCCCGCAAGGGCAAGCTGCCGCGCTTCGCCACCGTCAACGATCTGATG
GCCGATCTGCATGCGGCAGATTGA