Detailed information of TA system TA144513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-YefM
• Location: 32744..33260
• Replicon: plasmid pII
• Accession: NZ_CP046569
• Organism: Dietzia sp. DQ12-45-1b

Toxin (Protein)

• Gene name: relB
• Uniprot ID: A0A022L1N5
• Locus tag: GJR88_RS17695
• Protein ID: WP_017836491.1
• Coordinates: 33003..33260 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: GJR88_RS17690
• Protein ID: WP_063935029.1
• Coordinates: 32744..33001 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJR88_RS17670	30360..30596	+	237	Protein_26	hypothetical protein	-
GJR88_RS17675	30715..31227	+	513	WP_156607496.1	hypothetical protein	-
GJR88_RS17680	31306..31629	-	324	WP_063935027.1	hypothetical protein	-
GJR88_RS17685	31767..32345	-	579	WP_063935028.1	DNA-binding protein	-
GJR88_RS17690	32744..33001	+	258	WP_063935029.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
GJR88_RS17695	33003..33260	+	258	WP_017836491.1	Txe/YoeB family addiction module toxin	Toxin
GJR88_RS17700	33409..33744	+	336	WP_156607486.1	Lsr2 family protein	-
GJR88_RS17705	34267..34896	-	630	WP_156607488.1	TetR/AcrR family transcriptional regulator	-
GJR88_RS17710	35193..35717	+	525	WP_156607490.1	PaaI family thioesterase	-
GJR88_RS17715	35867..36868	-	1002	WP_082924073.1	AraC family transcriptional regulator	-
GJR88_RS17720	36987..37307	+	321	WP_067718080.1	2Fe-2S iron-sulfur cluster binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..46841	46841

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10369.76 Da
• Isoelectric Point: 8.0063

>T144513 WP_017836491.1 NZ_CP046569:33003-33260 [Dietzia sp. DQ12-45-1b]
VLLVWDDNAWQDYLWWQQQDRKILKRINLLLQDITRNGNEGIGKPEPLKYDFAGYWSRRITDEHRLVYKVTETEVRIATC
RYHYG

Nucleotide

• Length: 258 bp

>T144513 NZ_CP062115:c21191-20754 [Aminobacter sp. SR38]
GTGACCTTCCTGCTCGATGTTAATGTGCTGATCGCGCTGATCGATCCTGGCCATGTCGCCCATGACGACGCGCATGGCTG
GTTCAAAGCGACCGGTCACGCTGCATGGGCCACCTGTCCCGTCACCGAGAACGGCGTCATCCGCATCGTCGGCAATCCAA
AGTATCCCAATTCTCCCGGCTCGCCTGCTGTCGTCGCGCAGATCGTTACCAAGTTGCGAACCCTGCATGGACACAGCTTC
TGGCCCGACGATGTGAGCCTGGTTGGATCGGGCGACATCGATGCCGCAAAAATCCTGACCTCGGCACAGGTCACCGATAC
CTATCTTCTTGCGCTCGCCAAGACGCATGGAGGCCAACTGGCGAGTTTCGACCGCAAGCTGTCGACTGCGGCCGTGAAGG
GCGGCAAGTCCGCACTGCATCTCATCTCCGCAAGGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9682.83 Da
• Isoelectric Point: 4.6899

>AT144513 WP_063935029.1 NZ_CP046569:32744-33001 [Dietzia sp. DQ12-45-1b]
MKTMSYTESRARYAEVLDGVTNDREEVIITRAGHEPVVMVSLDDYESLRETAHLMRSPANARRLLDAMERLESGRGQARD
LLDTD

Nucleotide

• Length: 258 bp

>AT144513 NZ_CP062115:c21415-21188 [Aminobacter sp. SR38]
ATGAGAACGACACTTGCGATTGATGACGACGTCCTCGTCGCAGCCAAAGCGATGGCCCGGCAGCAGGATCGCAGCGTTGG
CGAGATCATCACCGATCTGGCGCGGCGCTCGCTGCGCCGTCCGCAAGGCGGCGGGGAGCGCAATGGCATTCCTCTGCTGT
CGCCCCGCCCGGATGCACCTCCTGTCACGCTTGAGATGGTCAATGCCTTGCGTGACGAACTGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A022L1N5