Detailed information of TA system TA144510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 246246..246879
• Replicon: chromosome
• Accession: NZ_CP046564
• Organism: Bacillus sp. N1-1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A0J6CT91
• Locus tag: GNK04_RS01360
• Protein ID: WP_048312992.1
• Coordinates: 246529..246879 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GNK04_RS01355
• Protein ID: WP_169525642.1
• Coordinates: 246246..246524 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNK04_RS01335	241757..242116	+	360	WP_159780892.1	holo-ACP synthase	-
GNK04_RS01340	242198..243703	+	1506	WP_205689156.1	NAD(P)H-hydrate dehydratase	-
GNK04_RS01345	243766..244767	+	1002	WP_159780894.1	outer membrane lipoprotein carrier protein LolA	-
GNK04_RS01350	244945..246111	+	1167	WP_159780895.1	alanine racemase	-
GNK04_RS01355	246246..246524	+	279	WP_169525642.1	antitoxin	Antitoxin
GNK04_RS01360	246529..246879	+	351	WP_048312992.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
GNK04_RS01365	247156..247977	+	822	WP_159780896.1	STAS domain-containing protein	-
GNK04_RS01370	247989..248345	+	357	WP_048312990.1	STAS domain-containing protein	-
GNK04_RS01375	248349..248750	+	402	WP_159780897.1	anti-sigma regulatory factor	-
GNK04_RS01380	248766..249776	+	1011	WP_159780898.1	PP2C family protein-serine/threonine phosphatase	-
GNK04_RS01385	249852..250181	+	330	WP_098446066.1	STAS domain-containing protein	-
GNK04_RS01390	250205..250660	+	456	WP_159786998.1	anti-sigma B factor RsbW	-
GNK04_RS01395	250629..251420	+	792	WP_098446067.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13002.09 Da
• Isoelectric Point: 6.4695

>T144510 WP_048312992.1 NZ_CP046564:246529-246879 [Bacillus sp. N1-1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDEGMMQRVQEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T144510 NZ_CP062074:c1241799-1241053 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTGAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10600.10 Da
• Isoelectric Point: 5.1695

>AT144510 WP_169525642.1 NZ_CP046564:246246-246524 [Bacillus sp. N1-1]
VSEANKKSIVVTLPQHILNEVDGIIQQEQLDRNEFISQATTMYIRERKKRQIRDAMRQGYMEMAKINLNLAAEAFLVEEE
AEHTVDRLVSGV

Nucleotide

• Length: 279 bp

>AT144510 NZ_CP062074:c1241052-1240882 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0J6CT91