Detailed information of TA system TA144498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1134447..1135066
• Replicon: chromosome
• Accession: NZ_CP046557
• Organism: Leclercia sp. 1106151

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A345CDT0
• Locus tag: GNG29_RS05345
• Protein ID: WP_032616864.1
• Coordinates: 1134447..1134665 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: GNG29_RS05350
• Protein ID: WP_103178503.1
• Coordinates: 1134674..1135066 (-)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNG29_RS05325	1131317..1131670	+	354	WP_103178500.1	DUF1428 family protein	-
GNG29_RS05330	1131703..1133271	-	1569	WP_103178501.1	EAL domain-containing protein	-
GNG29_RS05335	1133529..1133669	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
GNG29_RS05340	1133719..1134183	-	465	WP_156654006.1	hypothetical protein	-
GNG29_RS05345	1134447..1134665	-	219	WP_032616864.1	hemolysin expression modulator Hha	Toxin
GNG29_RS05350	1134674..1135066	-	393	WP_103178503.1	Hha toxicity modulator TomB	Antitoxin
GNG29_RS05355	1135556..1138696	-	3141	WP_103178504.1	multidrug efflux RND transporter permease subunit AcrB	-
GNG29_RS05360	1138719..1139912	-	1194	WP_156654007.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1124193..1135066	10873

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8583.95 Da
• Isoelectric Point: 8.9008

>T144498 WP_032616864.1 NZ_CP046557:c1134665-1134447 [Leclercia sp. 1106151]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Nucleotide

• Length: 219 bp

>T144498 NZ_CP062008:2405033-2405434 [Mycolicibacterium mucogenicum DSM 44124]
ATGATCGTGGACACATCGGCCATCGTCGCGCTGATTGCTGACGAGGACGACGTGGGAGTCTATGCCCGGGCGATCGCCGC
CGCAGTGGTGCCGCGGTTGTCGGCGGCAAGCTATCTCGAATGCGGAATCGTCCTGGATGCACGCCGTGATCCAGTCATCA
GTACGCGGTTCGATGACCTGGTGGATAGCGCCGGATTCGTCATCGAACCGGTGACCGAGCGTCAGGCCCGGCTGGCCCGC
CAGGCCTATCGTGACTTCGGGAAAGGCAGCGGCCACCCAGCGGGCCTGAACTTCGGTGACTGCTTCGCATATGCACTGGC
GATCGACTGCCGCGAGCCATTGCTGTGGAAGGGCGATGACTTCGGACACACGGGTGTCCGGTCAGCGCTCGAGTCCAGCT
AG

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 15150.05 Da
• Isoelectric Point: 4.8941

>AT144498 WP_103178503.1 NZ_CP046557:c1135066-1134674 [Leclercia sp. 1106151]
MDEYSPKRQDIAQLKFLCESLYHDCLTNLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNASRANPLASPIKIITNN

Nucleotide

• Length: 393 bp

>AT144498 NZ_CP062008:2404794-2405036 [Mycolicibacterium mucogenicum DSM 44124]
ATGGCGCTGAATATCAAAGACCCGGCCGTCCATGACGCCGTGAAAGAGATCGCAAGGATCACCGGCGAGTCGCAGGCGCA
GGCCGTCGCGACCGCGGTGAACGATCGGCTGCGACGATTGCAGGCCGATGATCTGGCAGAGCGCTTGCTGGATATTGCCC
GCAGTATCAGGAGCCGGCTGGGCCCCGAGGGACTTCCCGACCATGGCGAGCTGCTTTACGACGAATACGGACTGCCCAAA
TGA

Structures

Toxin

• AlphaFold DB: A0A345CDT0