Detailed information of TA system TA144493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 1773..2530
• Replicon: plasmid pFHI_NMBU_10_01
• Accession: NZ_CP046540
• Organism: Escherichia coli strain FHI_NMBU_10

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A0L6ZF90
• Locus tag: GOE21_RS26015
• Protein ID: WP_000501972.1
• Coordinates: 2045..2530 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: GOE21_RS26010
• Protein ID: WP_072141942.1
• Coordinates: 1773..2057 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GOE21_RS26000	1..858	+	858	WP_000131008.1	incFII family plasmid replication initiator RepA	-
GOE21_RS26005	1558..1698	+	141	WP_021503378.1	hypothetical protein	-
GOE21_RS26010	1773..2057	+	285	WP_072141942.1	DUF1778 domain-containing protein	Antitoxin
GOE21_RS26015	2045..2530	+	486	WP_000501972.1	GNAT family N-acetyltransferase	Toxin
GOE21_RS26020	2914..4124	+	1211	WP_172398176.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..72427	72427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-93)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17630.39 Da
• Isoelectric Point: 9.9107

>T144493 WP_000501972.1 NZ_CP046540:2045-2530 [Escherichia coli]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQALGATRTFVVCRKGTQQVVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDASFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTESAKQFYIHHGFTPSKTQLQTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T144493 NZ_CP061977:220852-221295 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10973.61 Da
• Isoelectric Point: 4.5625

>AT144493 WP_072141942.1 NZ_CP046540:1773-2057 [Escherichia coli]
MIYGGFMKSDVQLNLRARESQRILIDTAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADEP
AIEKLLARKPQWDV

Nucleotide

• Length: 285 bp

>AT144493 NZ_CP061977:220625-220855 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A0L6ZF90