Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 5290908..5291497 Replicon chromosome
Accession NZ_CP046539
Organism Escherichia coli strain FHI_NMBU_10

Toxin (Protein)


Gene name hicA Uniprot ID A0A398TMA1
Locus tag GOE21_RS25895 Protein ID WP_000513533.1
Coordinates 5290908..5291156 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A3J8R0E8
Locus tag GOE21_RS25900 Protein ID WP_000681611.1
Coordinates 5291153..5291497 (+) Length 115 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GOE21_RS25860 5286101..5286790 - 690 WP_000187063.1 YqaJ viral recombinase family protein -
GOE21_RS25865 5286787..5287737 - 951 WP_086257760.1 recombinase RecT -
GOE21_RS25870 5287755..5288036 - 282 WP_044191085.1 host nuclease inhibitor GamL -
GOE21_RS25875 5288057..5288338 - 282 WP_033805712.1 hypothetical protein -
GOE21_RS25880 5288350..5288562 - 213 WP_033805711.1 cell division inhibitor protein -
GOE21_RS25885 5288633..5289271 - 639 WP_044191088.1 antA/AntB antirepressor family protein -
GOE21_RS25890 5289538..5290089 - 552 WP_044191090.1 hypothetical protein -
GOE21_RS25895 5290908..5291156 + 249 WP_000513533.1 type II toxin-antitoxin system HicA family toxin Toxin
GOE21_RS25900 5291153..5291497 + 345 WP_000681611.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GOE21_RS25905 5291612..5291782 - 171 WP_164834428.1 hypothetical protein -
GOE21_RS25910 5291904..5292617 - 714 WP_044784361.1 LexA family transcriptional regulator -
GOE21_RS25915 5292713..5292916 + 204 WP_001240875.1 hypothetical protein -
GOE21_RS25920 5293450..5293836 + 387 WP_033885136.1 hypothetical protein -
GOE21_RS25925 5293830..5295332 + 1503 WP_086257762.1 DEAD/DEAH box helicase -
GOE21_RS25930 5295322..5296293 + 972 WP_033805703.1 toprim domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - rcsB / rcsB 5276745..5303603 26858


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9436.07 Da        Isoelectric Point: 10.3195

>T144492 WP_000513533.1 NZ_CP046539:5290908-5291156 [Escherichia coli]
MGKADKLLEKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Download         Length: 249 bp

>T144492 NZ_CP061977:92394-92678 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTCCATGGACGCAAAGACCAGTACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTAACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTGACCAAACACAGAAATTAA

Antitoxin


Download         Length: 115 a.a.        Molecular weight: 13065.80 Da        Isoelectric Point: 4.6791

>AT144492 WP_000681611.1 NZ_CP046539:5291153-5291497 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEASTLAELEQEFKTSVELYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVNHHTGA

Download         Length: 345 bp

>AT144492 NZ_CP061977:92156-92404 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACCTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCATATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A398TMA1

Antitoxin

Source ID Structure
AlphaFold DB A0A3J8R0E8

References