Detailed information of TA system TA144491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5028330..5028955
• Replicon: chromosome
• Accession: NZ_CP046539
• Organism: Escherichia coli strain FHI_NMBU_10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GOE21_RS24650
• Protein ID: WP_000911330.1
• Coordinates: 5028557..5028955 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: GOE21_RS24645
• Protein ID: WP_000450524.1
• Coordinates: 5028330..5028557 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GOE21_RS24620	5024133..5024603	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
GOE21_RS24625	5024603..5025175	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
GOE21_RS24630	5025321..5026199	+	879	WP_001345767.1	4-hydroxy-tetrahydrodipicolinate synthase	-
GOE21_RS24635	5026216..5027250	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
GOE21_RS24640	5027463..5028176	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
GOE21_RS24645	5028330..5028557	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GOE21_RS24650	5028557..5028955	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GOE21_RS24655	5029102..5029965	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
GOE21_RS24660	5029980..5031995	+	2016	WP_000829342.1	tRNA cytosine(34) acetyltransferase TmcA	-
GOE21_RS24665	5032069..5032767	+	699	WP_000679812.1	esterase	-
GOE21_RS24670	5032848..5033048	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T144491 WP_000911330.1 NZ_CP046539:5028557-5028955 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T144491 NZ_CP061977:c50074-49658 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT144491 WP_000450524.1 NZ_CP046539:5028330-5028557 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT144491 NZ_CP061977:c50301-50071 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33