Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3868435..3869066 Replicon chromosome
Accession NZ_CP046539
Organism Escherichia coli strain FHI_NMBU_10

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag GOE21_RS18900 Protein ID WP_001259388.1
Coordinates 3868435..3868710 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag GOE21_RS18905 Protein ID WP_000593555.1
Coordinates 3868707..3869066 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GOE21_RS18885 3863439..3864506 + 1068 WP_000361485.1 HlyD family secretion protein -
GOE21_RS18890 3864503..3867238 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
GOE21_RS18895 3867238..3868362 + 1125 WP_001314210.1 ABC transporter permease -
GOE21_RS18900 3868435..3868710 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
GOE21_RS18905 3868707..3869066 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GOE21_RS18910 3869186..3869587 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
GOE21_RS18915 3869593..3870399 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
GOE21_RS18920 3870396..3871160 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
GOE21_RS18925 3871160..3871993 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
GOE21_RS18930 3871990..3872934 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T144486 WP_001259388.1 NZ_CP046539:3868435-3868710 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T144486 NZ_CP061975:c4679840-4679556 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT144486 WP_000593555.1 NZ_CP046539:3868707-3869066 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT144486 NZ_CP061975:c4680072-4679830 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References