Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2160761..2161379 Replicon chromosome
Accession NZ_CP046539
Organism Escherichia coli strain FHI_NMBU_10

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GOE21_RS10900 Protein ID WP_001291435.1
Coordinates 2161161..2161379 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GOE21_RS10895 Protein ID WP_000344800.1
Coordinates 2160761..2161135 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GOE21_RS10885 2155850..2157043 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GOE21_RS10890 2157066..2160215 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GOE21_RS10895 2160761..2161135 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GOE21_RS10900 2161161..2161379 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GOE21_RS10905 2161550..2162101 + 552 WP_000102564.1 maltose O-acetyltransferase -
GOE21_RS10910 2162217..2162687 + 471 WP_000136192.1 YlaC family protein -
GOE21_RS10915 2162851..2164401 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GOE21_RS10920 2164443..2164796 - 354 WP_000878141.1 DUF1428 family protein -
GOE21_RS10930 2165175..2165486 + 312 WP_000409911.1 MGMT family protein -
GOE21_RS10935 2165517..2166089 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T144480 WP_001291435.1 NZ_CP046539:2161161-2161379 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T144480 NZ_CP046539:2161161-2161379 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT144480 WP_000344800.1 NZ_CP046539:2160761-2161135 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT144480 NZ_CP046539:2160761-2161135 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References