Detailed information of TA system TA144463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 899962..900602
• Replicon: chromosome
• Accession: NZ_CP046532
• Organism: Mannheimia sp. ZY171111

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: -
• Locus tag: GM698_RS04270
• Protein ID: WP_176809344.1
• Coordinates: 899962..900366 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: W0Q267
• Locus tag: GM698_RS04275
• Protein ID: WP_025235144.1
• Coordinates: 900363..900602 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GM698_RS04255 (GM698_04215)	898556..898858	-	303	WP_176812132.1	hypothetical protein	-
GM698_RS04260 (GM698_04220)	898873..899310	-	438	WP_176812131.1	hypothetical protein	-
GM698_RS04265 (GM698_04225)	899525..899785	+	261	WP_176807923.1	helix-turn-helix transcriptional regulator	-
GM698_RS04270 (GM698_04230)	899962..900366	-	405	WP_176809344.1	type II toxin-antitoxin system VapC family toxin	Toxin
GM698_RS04275 (GM698_04235)	900363..900602	-	240	WP_025235144.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GM698_RS04280 (GM698_04240)	900857..901222	-	366	WP_005621166.1	50S ribosomal protein L7/L12	-
GM698_RS04285 (GM698_04245)	901281..901772	-	492	WP_159628947.1	50S ribosomal protein L10	-
GM698_RS04290 (GM698_04250)	902052..902741	-	690	WP_176807921.1	50S ribosomal protein L1	-
GM698_RS04295 (GM698_04255)	902746..903174	-	429	WP_159628945.1	50S ribosomal protein L11	-
GM698_RS04300 (GM698_04260)	903320..903883	-	564	WP_176807920.1	transcription termination/antitermination protein NusG	-
GM698_RS04305 (GM698_04265)	903885..904298	-	414	WP_176807919.1	preprotein translocase subunit SecE	-
GM698_RS04310 (GM698_04270)	904421..905560	-	1140	WP_209289706.1	DNA-processing protein DprA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-116)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15121.67 Da
• Isoelectric Point: 7.6657

>T144463 WP_176809344.1 NZ_CP046532:c900366-899962 [Mannheimia sp. ZY171111]
MIYMLDTNILIYLMKNRPLAVAERVAQLTPSDQLVMSFITYAELLKGANGSANPEKALANIEKLKQRISVVYPNEKICEF
YGVWANKLKLQGKPIGGNDLWIACHALACNAILVTHNVKESERIEALNWQDWTC

Nucleotide

• Length: 405 bp

>T144463 NZ_CP061965:c5200439-5200056 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
AGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTACTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9108.09 Da
• Isoelectric Point: 3.9281

>AT144463 WP_025235144.1 NZ_CP046532:c900602-900363 [Mannheimia sp. ZY171111]
MRTKVFQSGNSQAVRIPFDFRFDVDTVEILQGQNGDVILRPVKPQADMEFLALFSDFDDDFIQALEDRDTSPPQERDDL

Nucleotide

• Length: 240 bp

>AT144463 NZ_CP061965:c5200681-5200439 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A7D5HSX4