Detailed information of TA system TA144461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1603237..1603836
• Replicon: chromosome
• Accession: NZ_CP046531
• Organism: Mannheimia ovis strain ZY170218

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GM695_RS07985
• Protein ID: WP_159631283.1
• Coordinates: 1603651..1603836 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GM695_RS07980
• Protein ID: WP_159631281.1
• Coordinates: 1603237..1603647 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GM695_RS07960	1599393..1600268	-	876	WP_159630267.1	4-hydroxybenzoate octaprenyltransferase	-
GM695_RS07965	1600261..1601022	-	762	WP_159630268.1	DeoR/GlpR family transcriptional regulator	-
GM695_RS07970	1601089..1602456	-	1368	WP_159630269.1	adenylosuccinate lyase	-
GM695_RS07975	1602490..1603119	-	630	WP_159630271.1	high frequency lysogenization protein HflD	-
GM695_RS07980	1603237..1603647	-	411	WP_159631281.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GM695_RS07985	1603651..1603836	-	186	WP_159631283.1	type II toxin-antitoxin system HicA family toxin	Toxin
GM695_RS07990	1603975..1605642	-	1668	WP_159630273.1	cysteine/glutathione ABC transporter ATP-binding protein/permease CydC	-
GM695_RS07995	1605642..1607408	-	1767	WP_159630275.1	cysteine/glutathione ABC transporter permease/ATP-binding protein CydD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6782.04 Da
• Isoelectric Point: 10.9862

>T144461 WP_159631283.1 NZ_CP046531:c1603836-1603651 [Mannheimia ovis]
IDSRSLVKLIVADGWFLVGIKGSHHQYKHPTKKGRVTIPHPKKDLPTATVKSILKQAAIKE

Nucleotide

• Length: 186 bp

>T144461 NZ_CP061965:c4715096-4714812 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATTTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGGGTCAGGTTATCGGTTGGTGCATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15169.24 Da
• Isoelectric Point: 4.3501

>AT144461 WP_159631281.1 NZ_CP046531:c1603647-1603237 [Mannheimia ovis]
MLYPIAIEMGNEKQAYGVIVPDVEGCFSAGDTLEEAFNNAKEAISFHIETLIEDGEAIPQPTALENHKNNPEYKDFIFAL
VDVDLTHLMGKTEKINVTLPSLLIRRIDEIVAQNPAYKTRSGFLAQVASERILTNY

Nucleotide

• Length: 411 bp

>AT144461 NZ_CP061965:c4715328-4715086 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA