Detailed information of TA system TA144460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3830675..3831360
• Replicon: chromosome
• Accession: NZ_CP046529
• Organism: Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF49
• Locus tag: AYJ03_RS18020
• Protein ID: WP_003917170.1
• Coordinates: 3830950..3831360 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AYJ03_RS18015
• Protein ID: WP_003912220.1
• Coordinates: 3830675..3830953 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ03_RS17995	3826664..3828265	-	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-
AYJ03_RS18000	3828282..3828986	-	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
AYJ03_RS18005	3829104..3829670	-	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
AYJ03_RS18010	3829732..3830619	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
AYJ03_RS18015	3830675..3830953	+	279	WP_003912220.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AYJ03_RS18020	3830950..3831360	+	411	WP_003917170.1	type II toxin-antitoxin system VapC family toxin	Toxin
AYJ03_RS18025	3831393..3833129	-	1737	WP_003418002.1	cholesterol oxidase	-
AYJ03_RS18030	3833185..3834312	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
AYJ03_RS18035	3834332..3835921	-	1590	WP_003900682.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14669.76 Da
• Isoelectric Point: 5.1784

>T144460 WP_003917170.1 NZ_CP046529:3830950-3831360 [Mycobacterium tuberculosis]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVESMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T144460 NZ_CP061965:c4620845-4620456 [Klebsiella pneumoniae]
ATGCAAACCAAATCATCCCCCCTCATGCGGGCGGCATCGTCACGTCCGTCTCCTGTGTATATCTGGCAGACCCTGCTGAC
CAGCCTGCTGGCACACCACTATGGCCTCACGCTTAGCGATACGCCGTTCATTGATGAACAGGTGATTCAGCAGCATATTG
ACGCGGGTATCTCGCTGGTTGATGCCCTGAATTTTATCGTTGAGAAGCATGAACTGGTCCGCACTGACCGGCCAGGCTTC
AGCATCCTGACGCAGTCTCCGTTTATCACACCCATAGATATCCTCCGGGCCAGGAAAGCAGCGGGGCTGATGAACCGTGA
CACGTATAAAGAGGTGACGGCTATTACCCGAGGGCAACATCCGCAGGTCAACACTTCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.75 Da
• Isoelectric Point: 10.1819

>AT144460 WP_003912220.1 NZ_CP046529:3830675-3830953 [Mycobacterium tuberculosis]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGRKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT144460 NZ_CP061965:c4621228-4620902 [Klebsiella pneumoniae]
ATCAGCGAACCCTGGTGGGGGCTCAGGCGCAGTGTCTCCCCGTGTTTTGGTGCCCGTCTGGTCCAGGAGGGAAACCACCT
GCATTACCTGGCTGACCGTGCGAATTTTAACGGACAGTTCTGCGATGCGGATTTGCGCCATCTTGACCAGGCTTTCCCGG
TATTGCTGAAACAGCTGGAGTTAATGCTTACCAGCGGTGAGCTGAATCCCCGTCATCAGCACGGCGTCACATTGTATGCA
AAAGGTCTGATCTGCGAGGCGGATACGCTCGGTTCGCATGGCTATGTTTATATCGCGATTTATCCCACTCCCGTCGCCAC
GGCATAA

Structures

Toxin

• AlphaFold DB: A5U883