Detailed information of TA system TA144458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3775166..3775695
• Replicon: chromosome
• Accession: NZ_CP046529
• Organism: Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: AYJ03_RS17760
• Protein ID: WP_003417760.1
• Coordinates: 3775438..3775695 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: AYJ03_RS17755
• Protein ID: WP_003417757.1
• Coordinates: 3775166..3775441 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ03_RS21115	3771964..3773401	-	1438	Protein_3470	FAD-binding oxidoreductase	-
AYJ03_RS17740	3773504..3773893	+	390	WP_003417745.1	DUF732 domain-containing protein	-
AYJ03_RS17745	3773907..3774200	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
AYJ03_RS17750	3774197..3775042	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
AYJ03_RS17755	3775166..3775441	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
AYJ03_RS17760	3775438..3775695	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
AYJ03_RS17765	3775737..3776927	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
AYJ03_RS17770	3777044..3777412	+	369	WP_003417765.1	FHA domain-containing protein	-
AYJ03_RS17775	3777409..3777960	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
AYJ03_RS17780	3777967..3778548	-	582	WP_003417769.1	ATP/GTP-binding protein	-
AYJ03_RS17785	3778529..3778897	-	369	WP_003417772.1	DUF742 domain-containing protein	-
AYJ03_RS17790	3778875..3779267	-	393	WP_003417776.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T144458 WP_003417760.1 NZ_CP046529:3775438-3775695 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T144458 NZ_CP061965:c4303389-4302904 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT144458 WP_003417757.1 NZ_CP046529:3775166-3775441 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT144458 NZ_CP061965:c4303679-4303386 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: G0TI94