Detailed information of TA system TA144450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3075340..3076027
• Replicon: chromosome
• Accession: NZ_CP046529
• Organism: Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: AYJ03_RS14550
• Protein ID: WP_003414064.1
• Coordinates: 3075632..3076027 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: AYJ03_RS14545
• Protein ID: WP_003414061.1
• Coordinates: 3075340..3075606 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ03_RS14520	3070979..3071881	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AYJ03_RS14525	3071950..3072702	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
AYJ03_RS14530	3072946..3073221	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
AYJ03_RS14535	3073218..3074840	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
AYJ03_RS14540	3074927..3075343	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
AYJ03_RS14545	3075340..3075606	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AYJ03_RS14550	3075632..3076027	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
AYJ03_RS14555	3076024..3076293	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
AYJ03_RS14560	3076303..3077397	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
AYJ03_RS14565	3077394..3077813	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
AYJ03_RS14570	3077887..3078366	-	480	WP_003414073.1	dihydrofolate reductase	-
AYJ03_RS14575	3078437..3079237	-	801	WP_003911953.1	thymidylate synthase	-
AYJ03_RS14580	3079393..3080130	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T144450 WP_003414064.1 NZ_CP046529:c3076027-3075632 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T144450 NZ_CP061962:207270-207554 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTCCATGGACGCAAAGACCAGTACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTAACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTGACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT144450 WP_003414061.1 NZ_CP046529:c3075606-3075340 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT144450 NZ_CP061962:207032-207280 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACCTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCATATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64