Detailed information of TA system TA144447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2936181..2936895
• Replicon: chromosome
• Accession: NZ_CP046529
• Organism: Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: AYJ03_RS13725
• Protein ID: WP_003413460.1
• Coordinates: 2936455..2936895 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: AYJ03_RS13720
• Protein ID: WP_003413456.1
• Coordinates: 2936181..2936468 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ03_RS13685	2931603..2931848	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
AYJ03_RS13690	2931845..2932249	+	405	WP_003899389.1	type II toxin-antitoxin system VapC family toxin	-
AYJ03_RS13695	2932466..2933086	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
AYJ03_RS13700	2933097..2933591	+	495	WP_003413444.1	DUF2617 family protein	-
AYJ03_RS13705	2933588..2934019	+	432	WP_003899390.1	DUF4247 domain-containing protein	-
AYJ03_RS13710	2934074..2934502	+	429	WP_178121172.1	DUF350 domain-containing protein	-
AYJ03_RS13715	2934499..2936070	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
AYJ03_RS13720	2936181..2936468	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
AYJ03_RS13725	2936455..2936895	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
AYJ03_RS13730	2936916..2937671	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
AYJ03_RS13735	2937804..2938400	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
AYJ03_RS13740	2938408..2939253	-	846	WP_003413466.1	acyl-CoA thioesterase II	-
AYJ03_RS13745	2939282..2940181	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
AYJ03_RS13750	2940309..2940983	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T144447 WP_003413460.1 NZ_CP046529:2936455-2936895 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T144447 NZ_CP061961:c5348686-5348303 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT144447 WP_003413456.1 NZ_CP046529:2936181-2936468 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT144447 NZ_CP061961:c5348928-5348686 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8