Detailed information of TA system TA144445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2874717..2875348
• Replicon: chromosome
• Accession: NZ_CP046529
• Organism: Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: AYJ03_RS13450
• Protein ID: WP_003413174.1
• Coordinates: 2874971..2875348 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: AYJ03_RS13445
• Protein ID: WP_003413167.1
• Coordinates: 2874717..2874974 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ03_RS13415	2870631..2870945	+	315	WP_009937839.1	hypothetical protein	-
AYJ03_RS13420	2871241..2872452	+	1212	WP_009936235.1	alpha/beta hydrolase family protein	-
AYJ03_RS13425	2872579..2873238	+	660	WP_003900846.1	LppA family lipoprotein	-
AYJ03_RS13430	2873235..2873897	+	663	WP_003900848.1	hypothetical protein	-
AYJ03_RS13435	2873894..2874172	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
AYJ03_RS13440	2874265..2874678	+	414	WP_003413164.1	PIN domain nuclease	-
AYJ03_RS13445	2874717..2874974	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
AYJ03_RS13450	2874971..2875348	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
AYJ03_RS13455	2875364..2875738	-	375	WP_003413177.1	hypothetical protein	-
AYJ03_RS13460	2875838..2876233	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
AYJ03_RS13465	2876230..2876475	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
AYJ03_RS13470	2876886..2877305	-	420	WP_003413190.1	A24 family peptidase	-
AYJ03_RS13475	2877317..2878126	-	810	WP_003413193.1	shikimate dehydrogenase	-
AYJ03_RS13480	2878123..2879376	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
AYJ03_RS13485	2879369..2879881	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T144445 WP_003413174.1 NZ_CP046529:2874971-2875348 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T144445 NZ_CP061961:c4833748-4833464 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT144445 WP_003413167.1 NZ_CP046529:2874717-2874974 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT144445 NZ_CP061961:c4833980-4833738 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5