Detailed information of TA system TA144443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2860378..2861018
• Replicon: chromosome
• Accession: NZ_CP046529
• Organism: Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: AYJ03_RS13355
• Protein ID: WP_003412970.1
• Coordinates: 2860378..2860797 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: AYJ03_RS13360
• Protein ID: WP_003412975.1
• Coordinates: 2860794..2861018 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ03_RS13330	2855963..2856685	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
AYJ03_RS13335	2857202..2857429	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
AYJ03_RS13340	2857426..2857827	+	402	WP_003412963.1	PIN domain-containing protein	-
AYJ03_RS13345	2857862..2858782	-	921	WP_003412965.1	restriction endonuclease	-
AYJ03_RS20815	2859123..2859368	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
AYJ03_RS13350	2859427..2860377	+	951	WP_003911916.1	Lsr2 family protein	-
AYJ03_RS13355	2860378..2860797	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
AYJ03_RS13360	2860794..2861018	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
AYJ03_RS13365	2861049..2863892	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
AYJ03_RS13370	2863964..2864365	-	402	WP_003412981.1	hypothetical protein	-
AYJ03_RS13375	2864365..2864835	-	471	WP_003899364.1	transcription antitermination factor NusB	-
AYJ03_RS13380	2864838..2865401	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T144443 WP_003412970.1 NZ_CP046529:c2860797-2860378 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T144443 NZ_CP061961:c4707090-4706557 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTTTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT144443 WP_003412975.1 NZ_CP046529:c2861018-2860794 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT144443 NZ_CP061961:c4707367-4707101 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09