Detailed information of TA system TA144432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2199112..2199815
• Replicon: chromosome
• Accession: NZ_CP046529
• Organism: Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: AYJ03_RS10205
• Protein ID: WP_003409778.1
• Coordinates: 2199112..2199441 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: AYJ03_RS10210
• Protein ID: WP_003409780.1
• Coordinates: 2199438..2199815 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ03_RS10185	2195495..2196565	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
AYJ03_RS10190	2196562..2197077	+	516	WP_003409718.1	flavin reductase family protein	-
AYJ03_RS10195	2197074..2198135	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
AYJ03_RS10200	2198132..2198902	+	771	WP_003409775.1	SDR family oxidoreductase	-
AYJ03_RS10205	2199112..2199441	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AYJ03_RS10210	2199438..2199815	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
AYJ03_RS10215	2199812..2200402	-	591	WP_003409784.1	SEC-C domain-containing protein	-
AYJ03_RS10220	2200457..2201821	+	1365	WP_003903691.1	HNH endonuclease	-
AYJ03_RS10225	2201976..2202428	-	453	WP_003899095.1	lipoprotein	-
AYJ03_RS10230	2202492..2202893	+	402	WP_003409869.1	hypothetical protein	-
AYJ03_RS10235	2202886..2203068	-	183	WP_003409870.1	hypothetical protein	-
AYJ03_RS10240	2203182..2203532	-	351	WP_003899096.1	hypothetical protein	-
AYJ03_RS10245	2203543..2204445	-	903	WP_003899097.1	hypothetical protein	-
AYJ03_RS10250	2204466..2204657	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T144432 WP_003409778.1 NZ_CP046529:c2199441-2199112 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T144432 NZ_CP061957:c5360530-5360147 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT144432 WP_003409780.1 NZ_CP046529:c2199815-2199438 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT144432 NZ_CP061957:c5360772-5360530 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8