Detailed information of TA system TA144424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1383256..1383944
• Replicon: chromosome
• Accession: NZ_CP046529
• Organism: Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: AYJ03_RS06570
• Protein ID: WP_003406304.1
• Coordinates: 1383513..1383944 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: AYJ03_RS06565
• Protein ID: WP_003406302.1
• Coordinates: 1383256..1383516 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ03_RS06545	1378833..1379657	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
AYJ03_RS06550	1379662..1380843	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
AYJ03_RS06555	1380920..1382020	-	1101	WP_003916789.1	magnesium/cobalt transporter CorA	-
AYJ03_RS06560	1382191..1383180	+	990	WP_003406301.1	malate dehydrogenase	-
AYJ03_RS06565	1383256..1383516	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
AYJ03_RS06570	1383513..1383944	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
AYJ03_RS06575	1383967..1385655	-	1689	WP_003910308.1	PE family protein	-
AYJ03_RS06580	1385835..1386695	+	861	WP_003900301.1	hypothetical protein	-
AYJ03_RS06585	1386776..1387606	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
AYJ03_RS06590	1387663..1387956	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
AYJ03_RS06595	1387953..1388222	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T144424 WP_003406304.1 NZ_CP046529:1383513-1383944 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T144424 NZ_CP061957:743840-744325 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT144424 WP_003406302.1 NZ_CP046529:1383256-1383516 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT144424 NZ_CP061957:743550-743843 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6