Detailed information of TA system TA144423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1238393..1238961
• Replicon: chromosome
• Accession: NZ_CP046529
• Organism: Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: AYJ03_RS05905
• Protein ID: WP_003405865.1
• Coordinates: 1238587..1238961 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: AYJ03_RS05900
• Protein ID: WP_003405863.1
• Coordinates: 1238393..1238590 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ03_RS05880	1234434..1235072	-	639	WP_003898730.1	lipid droplet-associated protein	-
AYJ03_RS05885	1235162..1236169	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
AYJ03_RS05890	1236186..1237169	-	984	WP_003898731.1	hypothetical protein	-
AYJ03_RS05895	1237232..1238305	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
AYJ03_RS05900	1238393..1238590	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AYJ03_RS05905	1238587..1238961	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
AYJ03_RS05910	1239164..1239862	+	699	WP_003898733.1	hypothetical protein	-
AYJ03_RS05915	1239980..1240165	+	186	WP_003901093.1	hypothetical protein	-
AYJ03_RS05920	1240092..1240367	-	276	WP_003405867.1	hypothetical protein	-
AYJ03_RS05925	1240610..1240933	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
AYJ03_RS05930	1240948..1241658	-	711	WP_003911399.1	hypothetical protein	-
AYJ03_RS20940	1241680..1241889	+	210	WP_003911400.1	hypothetical protein	-
AYJ03_RS05935	1241841..1242548	-	708	Protein_1159	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T144423 WP_003405865.1 NZ_CP046529:1238587-1238961 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T144423 NZ_CP061957:357649-357996 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT144423 WP_003405863.1 NZ_CP046529:1238393-1238590 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT144423 NZ_CP061957:357996-358295 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07