144422

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1229637..1230268 Replicon chromosome
Accession NZ_CP046529
Organism Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag AYJ03_RS05850 Protein ID WP_003898728.1
Coordinates 1229637..1229948 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag AYJ03_RS05855 Protein ID WP_003405836.1
Coordinates 1229948..1230268 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYJ03_RS05830 1225118..1226542 - 1425 WP_003405805.1 class II fumarate hydratase -
AYJ03_RS05835 1226573..1227661 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
AYJ03_RS05840 1227717..1228361 + 645 WP_003915494.1 DUF4245 domain-containing protein -
AYJ03_RS05845 1228368..1229525 - 1158 WP_003898727.1 AI-2E family transporter -
AYJ03_RS05850 1229637..1229948 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
AYJ03_RS05855 1229948..1230268 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
AYJ03_RS05860 1230278..1231803 + 1526 Protein_1143 carboxylesterase/lipase family protein -
AYJ03_RS05865 1231821..1232933 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
AYJ03_RS05870 1232943..1233200 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
AYJ03_RS05875 1233190..1234437 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
AYJ03_RS05880 1234434..1235072 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T144422 WP_003898728.1 NZ_CP046529:c1229948-1229637 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T144422 NZ_CP061957:331678-332046 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT144422 WP_003405836.1 NZ_CP046529:c1230268-1229948 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT144422 NZ_CP061957:331460-331681 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References