Detailed information of TA system TA144419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1013657..1014276
• Replicon: chromosome
• Accession: NZ_CP046529
• Organism: Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: AYJ03_RS04815
• Protein ID: WP_003404726.1
• Coordinates: 1013842..1014276 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: AYJ03_RS04810
• Protein ID: WP_003898641.1
• Coordinates: 1013657..1013836 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ03_RS04800	1009130..1010710	+	1581	WP_003909313.1	serine hydrolase	-
AYJ03_RS04805	1010707..1013100	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
AYJ03_RS20895	1013373..1013531	+	159	WP_003404720.1	hypothetical protein	-
AYJ03_RS04810	1013657..1013836	+	180	WP_003898641.1	antitoxin	Antitoxin
AYJ03_RS04815	1013842..1014276	+	435	WP_003404726.1	SRPBCC family protein	Toxin
AYJ03_RS04820	1014374..1015147	+	774	WP_003404735.1	VOC family protein	-
AYJ03_RS04825	1015212..1015661	+	450	WP_003404738.1	hypothetical protein	-
AYJ03_RS04830	1015796..1016026	-	231	WP_003898642.1	hypothetical protein	-
AYJ03_RS04835	1016193..1017701	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
AYJ03_RS04840	1017703..1018941	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T144419 WP_003404726.1 NZ_CP046529:1013842-1014276 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T144419 NZ_CP061952:c5281666-5281283 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT144419 WP_003898641.1 NZ_CP046529:1013657-1013836 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT144419 NZ_CP061952:c5281908-5281666 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5