Detailed information of TA system TA144410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 703573..704216
• Replicon: chromosome
• Accession: NZ_CP046529
• Organism: Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: AYJ03_RS03190
• Protein ID: WP_003403187.1
• Coordinates: 703815..704216 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: AYJ03_RS03185
• Protein ID: WP_003403184.1
• Coordinates: 703573..703818 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ03_RS03150	698853..699383	-	531	WP_003917320.1	two component system sensor kinase HK2	-
AYJ03_RS03155	699367..700062	-	696	WP_003918099.1	two-component system response regulator TcrA	-
AYJ03_RS03160	700185..700496	+	312	WP_003403164.1	hypothetical protein	-
AYJ03_RS03165	700568..701518	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
AYJ03_RS03170	701759..702343	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
AYJ03_RS03175	702345..703055	+	711	Protein_625	transposase	-
AYJ03_RS03180	703058..703528	+	471	WP_003898523.1	hypothetical protein	-
AYJ03_RS03185	703573..703818	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AYJ03_RS03190	703815..704216	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
AYJ03_RS03200	704604..704771	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
AYJ03_RS03205	704804..705001	-	198	WP_003403191.1	hypothetical protein	-
AYJ03_RS03210	705081..706238	-	1158	WP_003898524.1	hypothetical protein	-
AYJ03_RS03215	706290..706511	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
AYJ03_RS03220	706653..707258	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T144410 WP_003403187.1 NZ_CP046529:703815-704216 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T144410 NZ_CP061952:739081-739566 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT144410 WP_003403184.1 NZ_CP046529:703573-703818 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT144410 NZ_CP061952:738791-739084 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91