Detailed information of TA system TA144409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 697483..698129
• Replicon: chromosome
• Accession: NZ_CP046529
• Organism: Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: AYJ03_RS03135
• Protein ID: WP_003403137.1
• Coordinates: 697483..697896 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: AYJ03_RS03140
• Protein ID: WP_003403139.1
• Coordinates: 697893..698129 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ03_RS03115	693566..695116	+	1551	WP_003403119.1	MCE family protein	-
AYJ03_RS03120	695168..695560	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
AYJ03_RS03125	695557..695814	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
AYJ03_RS03130	695997..697232	-	1236	WP_003403128.1	ATP-binding protein	-
AYJ03_RS03135	697483..697896	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
AYJ03_RS03140	697893..698129	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AYJ03_RS03145	698233..698739	-	507	WP_003900973.1	two component system sensor kinase HK1	-
AYJ03_RS03150	698853..699383	-	531	WP_003917320.1	two component system sensor kinase HK2	-
AYJ03_RS03155	699367..700062	-	696	WP_003918099.1	two-component system response regulator TcrA	-
AYJ03_RS03160	700185..700496	+	312	WP_003403164.1	hypothetical protein	-
AYJ03_RS03165	700568..701518	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
AYJ03_RS03170	701759..702343	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
AYJ03_RS03175	702345..703055	+	711	Protein_625	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T144409 WP_003403137.1 NZ_CP046529:c697896-697483 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T144409 NZ_CP061952:352890-353237 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT144409 WP_003403139.1 NZ_CP046529:c698129-697893 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT144409 NZ_CP061952:353237-353536 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89