Detailed information of TA system TA144403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 363802..364373
• Replicon: chromosome
• Accession: NZ_CP046529
• Organism: Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: AYJ03_RS01590
• Protein ID: WP_003401560.1
• Coordinates: 363802..364104 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: AYJ03_RS01595
• Protein ID: WP_003401563.1
• Coordinates: 364152..364373 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ03_RS01570	359271..360074	-	804	WP_003401540.1	hypothetical protein	-
AYJ03_RS01575	360084..361481	-	1398	WP_003401544.1	sulfatase	-
AYJ03_RS01580	361660..363435	+	1776	WP_010886074.1	PE family protein	-
AYJ03_RS01585	363578..363805	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
AYJ03_RS01590	363802..364104	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
AYJ03_RS01595	364152..364373	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
AYJ03_RS01600	364370..364795	+	426	WP_003401566.1	PIN domain nuclease	-
AYJ03_RS01605	364931..365563	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
AYJ03_RS01610	365560..366468	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T144403 WP_003401560.1 NZ_CP046529:363802-364104 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T144403 NZ_CP061948:c4808922-4808638 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT144403 WP_003401563.1 NZ_CP046529:364152-364373 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT144403 NZ_CP061948:c4809154-4808912 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A829CG48