Detailed information of TA system TA144392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 4724439..4724910
• Replicon: chromosome
• Accession: NZ_CP046527
• Organism: Escherichia coli strain RM19259

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: GN312_RS23660
• Protein ID: WP_001303511.1
• Coordinates: 4724632..4724910 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: GN312_RS23655
• Protein ID: WP_001302048.1
• Coordinates: 4724439..4724630 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN312_RS23615	4719646..4720230	-	585	WP_000206793.1	DUF551 domain-containing protein	-
GN312_RS23620	4720287..4720682	-	396	WP_001118159.1	DUF977 family protein	-
GN312_RS23625	4720698..4721467	-	770	Protein_4499	DUF1627 domain-containing protein	-
GN312_RS23630	4721493..4722233	-	741	WP_000788938.1	ATP-binding protein	-
GN312_RS23635	4722240..4723202	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
GN312_RS23640	4723225..4723650	-	426	WP_000693943.1	Rha family transcriptional regulator	-
GN312_RS23645	4723647..4723949	-	303	WP_000172738.1	transcriptional regulator	-
GN312_RS23650	4724047..4724418	+	372	WP_001169686.1	hypothetical protein	-
GN312_RS23655	4724439..4724630	+	192	WP_001302048.1	hypothetical protein	Antitoxin
GN312_RS23660	4724632..4724910	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GN312_RS23665	4725263..4725562	+	300	WP_001240334.1	hypothetical protein	-
GN312_RS23670	4725634..4725852	+	219	WP_001171930.1	hypothetical protein	-
GN312_RS23680	4726421..4726609	+	189	WP_000449192.1	cell division inhibition protein DicB	-
GN312_RS23685	4726606..4726797	+	192	WP_001090200.1	DUF1482 family protein	-
GN312_RS23690	4726890..4727975	+	1086	Protein_4511	exonuclease	-
GN312_RS23695	4728026..4729239	+	1214	WP_162829202.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	4642034..4741103	99069

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T144392 WP_001303511.1 NZ_CP046527:4724632-4724910 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T144392 NZ_CP061944:c5137011-5136712 [Pseudomonas sp. VLB120]
ATGACCTATAGCCTTGAGTTCGATGCGCGAGCGCTAAAGGAATGGCAAAAGCTGGGGGATACGGTCCGGCAGCAACTCAA
GAAAAAACTCGCCTCGGTTTTGCTCAATCCGAGGGTTGAGGCGAACCGGCTGCATAGCTTGCCGGACTGCTACAAGATCA
AGCTGCGCAGTAGCGGCTACCGATTGGTCTACCAGGTGATCGAACAAGAGGTTGTAGTTTTTGTGGTAGCTGTGGATAAG
CGCGAGCGGGAACAGGCTTATCTTAAGGCGGCAGAGCGAGTGGTAGGTAAAACGTCATAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT144392 WP_001302048.1 NZ_CP046527:4724439-4724630 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT144392 NZ_CP061944:c5137240-5137001 [Pseudomonas sp. VLB120]
ATGGCATCCATCAATGTTCGTATCGACGACGATCTCAAAATCCGAGCCTATCGTGAGCTTGAGCGGTTGGGTGTTACGCC
CTCCGAGCTCATGCGCCAAGCCTTGCAGTACGTCGCCGAGCGCGGGCAGTTGCCTTTTAAGCCTGTGCTCATGACTGATG
AAGATGAGGCGCTGCTAGCGATCGTTCGCGAGCGCCTTGCCGACCCTCAGCGAGTGAGGGTTTCGCTGGATGACCTATAG

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0